中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 地质与地球物理研究所
  3. 中国科学院地质与地球物理研究所
  4. 中国科学院地球与行星物理重点实验室
Adaptive mechanisms of Bacillus to near space extreme environments

文献类型：期刊论文

作者Deng, Aihua1; Wang, Tiantian1,5; Wang, Junyue1,5; Li, Lai1,5; Wang, Xueliang1,5; Liu, Li4,5; Wen, Tingyi1,2,3
刊名SCIENCE OF THE TOTAL ENVIRONMENT
出版日期2023-08-15
卷号886页码:14
ISSN号0048-9697
关键词Near space Bacillus Genome Transcriptome Proteome Adaptive mechanism
DOI10.1016/j.scitotenv.2023.163952
英文摘要Earth's near space is an extreme atmosphere environment with high levels of radiation, low atmospheric pressure and dramatic temperature fluctuations. The region is above the flight altitude of aircraft but below the orbit of satellites, which has special and Mars-like conditions for investigating the survival and evolution of life. Technical limitations including flight devices, payloads and technologies/methodologies hinder microbiological research in near space. In this study, we investigated microbial survival and adaptive strategies in near space using a scientific balloon fight mission and multi-omics analyses. Methods for sample preparation, storage, protector and vessel were optimized to prepare the exposed microbial samples. After 3 h 17 min of exposure at a float altitude of similar to 32 km, only Bacillus strains were alive with survival efficiencies of 0-10-6. Diverse mutants with significantly altered metabolites were generated, firstly proving that Earth's near space could be used as a new powerful microbial breeding platform. Multi-omics analyses of mutants revealed cascade changes at the genome, transcriptome and proteome levels. In response to environmental stresses, two mutants had similar proteome changes caused by different genomic mutations and mRNA expression levels. Metabolic network analysis combined with proteins' expression levels revealed that metabolic fluxes of EMP, PPP and purine synthesis-related pathways were significantly altered to increase/decrease inosine production. Further analysis showed that proteins related to translation, molecular chaperones, cell wall/membrane, sporulation, DNA replication/repair and anti-oxidation were significantly upregulated, enabling cells to efficiently repair DNA/protein damages and improve viability against environmental stress. Overall, these results revealed genetic and metabolic responses of Bacillus to the harsh conditions in near space, providing a research basis for bacterial adaptive mechanisms in extreme environments.
WOS关键词PURINE NUCLEOSIDES ; EARTHS ; SURVIVAL ; SUBTILIS ; BIOSYNTHESIS ; MUTATIONS ; EXPOSURE ; BACTERIA ; ALTITUDE ; GROWTH
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA17010503] ; National Natural Science Foundation of China[31570083] ; National Natural Science Foundation of China[31870070] ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences[IAGM-2019-A02]
WOS研究方向Environmental Sciences & Ecology
语种英语
出版者ELSEVIER
WOS记录号WOS:001001463400001
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences ; Innovation Academy for Green Manufacture, Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/111205]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Deng, Aihua; Wen, Tingyi
作者单位1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
2.Chinese Acad Sci, China Innovat Acad Green Manufacture, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Deng, Aihua,Wang, Tiantian,Wang, Junyue,et al. Adaptive mechanisms of Bacillus to near space extreme environments[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,886:14.
APA Deng, Aihua.,Wang, Tiantian.,Wang, Junyue.,Li, Lai.,Wang, Xueliang.,...&Wen, Tingyi.(2023).Adaptive mechanisms of Bacillus to near space extreme environments.SCIENCE OF THE TOTAL ENVIRONMENT,886,14.
MLA Deng, Aihua,et al."Adaptive mechanisms of Bacillus to near space extreme environments".SCIENCE OF THE TOTAL ENVIRONMENT 886(2023):14.

入库方式： OAI收割

来源：地质与地球物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。