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Effects of static magnetic field on the sulfate metabolic pathway involved in Magnetospirillum magneticum AMB-1 cell growth and magnetosome formation

文献类型：期刊论文

作者Chen, Haitao1,7; Shi, Hongkai1,3,7; Chen, Changyou1,7; Jiao, Yangkun1,3,7; Wang, Pingping1,7; Chen, Chuanfang1,7; Li, Jinhua5,7; Wu, Long-Fei4,6; Song, Tao1,2,3,7
刊名JOURNAL OF APPLIED MICROBIOLOGY
出版日期2023-12-01
卷号134期号:12页码:14
ISSN号1364-5072
关键词static magnetic field magnetosomes transcriptomic analysis sulfur metabolism reactive oxidative species
DOI10.1093/jambio/lxad302
英文摘要Aims Magnetotactic bacteria (MTB) can use their unique intracellular magnetosome organelles to swim along the Earth's magnetic field. They play important roles in the biogeochemical cycles of iron and sulfur. Previous studies have shown that the applied magnetic fields could affect the magnetosome formation and antioxidant defense systems in MTB. However, the molecular mechanisms by which magnetic fields affect MTB cells remain unclear. We aim to better understand the dark at 28 degrees C-29 degrees C for 20 h, as shownthe interactions between magnetic fields and cells, and the mechanism of MTB adaptation to magnetic field at molecular levels.Methods and results We performed microbiological, transcriptomic, and genetic experiments to analyze the effects of a weak static magnetic field (SMF) exposure on the cell growth and magnetosome formation in the MTB strain Magnetospirillum magneticum AMB-1. The results showed that a 1.5 mT SMF significantly promoted the cell growth but reduced magnetosome formation in AMB-1, compared to the geomagnetic field. Transcriptomic analysis revealed decreased expression of genes primarily involved in the sulfate reduction pathway. Consistently, knockout mutant lacking adenylyl-sulfate kinase CysC did no more react to the SMF and the differences in growth and Cmag disappeared. Together with experimental findings of increased reactive oxidative species in the SMF-treated wild-type strain, we proposed that cysC, as a key gene, can participate in the cell growth and mineralization in AMB-1 by SMF regulation.Conclusions This study suggests that the magnetic field exposure can trigger a bacterial oxidative stress response involved in AMB-1 growth and magnetosome mineralization by regulating the sulfur metabolism pathway. CysC may serve as a pivotal enzyme in mediating sulfur metabolism to synchronize the impact of SMF on both growth and magnetization of AMB-1.
WOS关键词MAGNETOTACTIC BACTERIA ; ESCHERICHIA-COLI ; GRYPHISWALDENSE ; BIOMINERALIZATION ; DENITRIFICATION ; BIOGENESIS ; PROTEIN
资助项目National Natural Science Foundation of China[51937011] ; National Natural Science Foundation of China[32100031] ; National Natural Science Foundation of China[32071237] ; National Natural Science Foundation of China[51907192]
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
出版者OXFORD UNIV PRESS
WOS记录号WOS:001131517400001
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.iggcas.ac.cn/handle/132A11/111680]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Song, Tao
作者单位1.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Biol Electromagnetism, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Bioelectromagnetism, 6 Beier Tiao Zhongguancun HaiDian, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Elect Elect & Commun Engn, Beijing 100049, Peoples R China
4.CNRS, France China Int Lab Evolut & Dev Magnetotact Mult, F-13402 Marseille, France
5.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China
6.Aix Marseille Univ, CNRS, LCB, F-13402 Marseille, France
7.Chinese Acad Sci, France China Int Lab Evolut & Dev Magnetotact Mult, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Haitao,Shi, Hongkai,Chen, Changyou,et al. Effects of static magnetic field on the sulfate metabolic pathway involved in Magnetospirillum magneticum AMB-1 cell growth and magnetosome formation[J]. JOURNAL OF APPLIED MICROBIOLOGY,2023,134(12):14.
APA Chen, Haitao.,Shi, Hongkai.,Chen, Changyou.,Jiao, Yangkun.,Wang, Pingping.,...&Song, Tao.(2023).Effects of static magnetic field on the sulfate metabolic pathway involved in Magnetospirillum magneticum AMB-1 cell growth and magnetosome formation.JOURNAL OF APPLIED MICROBIOLOGY,134(12),14.
MLA Chen, Haitao,et al."Effects of static magnetic field on the sulfate metabolic pathway involved in Magnetospirillum magneticum AMB-1 cell growth and magnetosome formation".JOURNAL OF APPLIED MICROBIOLOGY 134.12(2023):14.

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来源：地质与地球物理研究所

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