中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains

文献类型：期刊论文

作者Wang, Jingyi8,9,10; Lin, Jianbang8,9; Chen, Yefei9; Liu, Jing7,9; Zheng, Qiongping9; Deng, Mao6,9; Wang, Ruiqi9; Zhang, Yujing9; Feng, Shijing9; Xu, Zhenyan9
刊名CELL REPORTS
出版日期2023-11-28
卷号42期号:11页码:21
ISSN号2211-1247
DOI10.1016/j.celrep.2023.113348
通讯作者Chen, Qian(chenqian@simm.ac.cn) ; Lu, Zhonghua(zh.lu@siat.ac.cn)
英文摘要Promoters are essential tools for basic and translational neuroscience research. An ideal promoter should possess the shortest possible DNA sequence with cell-type selectivity. However, whether ultra-compact pro-moters can offer neuron-specific expression is unclear. Here, we report the development of an extremely short promoter that enables selective gene expression in neurons, but not glial cells, in the brain. The pro-moter sequence originates from the human CALM1 gene and is only 120 bp in size. The CALM1 promoter (pCALM1) embedded in an adeno-associated virus (AAV) genome directed broad reporter expression in excitatory and inhibitory neurons in mouse and monkey brains. Moreover, pCALM1, when inserted into an all-in-one AAV vector expressing SpCas9 and sgRNA, drives constitutive and conditional in vivo gene editing in neurons and elicits functional alterations. These data demonstrate the ability of pCALM1 to conduct restricted neuronal gene expression, illustrating the feasibility of ultra-miniature promoters for targeting brain-cell subtypes.
WOS关键词AUTISTIC-LIKE BEHAVIORS ; ADENOASSOCIATED VIRUS ; TRANSGENE EXPRESSION ; GENE DELIVERY ; VECTOR ; RECOMBINATION ; OPPORTUNITIES ; INTERNEURONS ; CHALLENGES ; VARIANTS
资助项目Ministry of Science and Technology of the People's Republic of China[2018YFA0801404] ; Ministry of Science and Technology of the People's Republic of China[2019YFA0903803] ; National Natural Science Foundation of China[81961128019] ; National Natural Science Foundation of China[31871090] ; National Natural Science Foundation of China[32000730] ; National Natural Science Foundation of China[82271578] ; National Natural Science Foundation of China[81871078] ; Shenzhen Science and Technology Innovation Commission[KQTD20210811090117032] ; Shenzhen Science and Technology Innovation Commission[JCYJ202103243001018] ; Shenzhen Science and Technology Innovation Commission[JCYJ20190809171003698] ; Shanghai Pujiang program[22PJ1415500] ; Zhongshan Bureau of Science and Technology grant[CXTD2022013] ; Guangdong Province High -level Innovative Research Institute[2021B0909050003] ; China Postdoctoral Science Foundation[2019M653115]
WOS研究方向Cell Biology
语种英语
出版者CELL PRESS
WOS记录号WOS:001108850400001
源URL[http://119.78.100.183/handle/2S10ELR8/308107]  
专题中国科学院上海药物研究所
通讯作者Chen, Qian; Lu, Zhonghua
作者单位1.Chinese Acad Sci, Biomed Imaging Sci & Syst Key Lab, Shenzhen 518055, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
3.Hubei Univ Med, Biomed Res Inst, Shiyan 442000, Peoples R China
4.Chinese Acad Sci, Guangdong Prov Key Lab Brain Connectome & Behav, CAS Key Lab Brain Connectome & Manipulat, Brain Cognit & Brain Dis Inst,Shenzhen Inst Adv Te, Shenzhen 518055, Peoples R China
5.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China
6.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China
7.Southern Med Univ, Affiliated Shenzhen Matern & Child Healthcare Hosp, Dept Neonatol, Shenzhen 518027, Peoples R China
8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
9.Chinese Acad Sci, Shenzhen Hong Kong Inst Brain Sci, Shenzhen Inst Adv Technol, Shenzhen Technol Res Ctr Primate Translat Med,Shen, Shenzhen 518055, Peoples R China
10.Peking Univ, Shenzhen Hosp, Dept Anesthesiol, Shenzhen 518034, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Jingyi,Lin, Jianbang,Chen, Yefei,et al. An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains[J]. CELL REPORTS,2023,42(11):21.
APA Wang, Jingyi.,Lin, Jianbang.,Chen, Yefei.,Liu, Jing.,Zheng, Qiongping.,...&Lu, Zhonghua.(2023).An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains.CELL REPORTS,42(11),21.
MLA Wang, Jingyi,et al."An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains".CELL REPORTS 42.11(2023):21.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。