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Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.

文献类型：期刊论文

作者Huang, Yanan1,2; Liu, Helu1; Zhou, Yang1; Lu, Zaiqing3; Pu, Yujin1,2; Zhang, Haibin1
刊名GENE
出版日期2024-02-05
卷号894页码:9
ISSN号0378-1119
关键词Deep-sea Holothurian Chiridota sp Sea cucumber triterpenes GC -MS
DOI10.1016/j.gene.2023.147971
通讯作者Zhang, Haibin(hzhang@idsse.ac.cn)
英文摘要Saponins derived from holothurians have high potential medicinal value. However, the de novo synthesis of the derivatization of triterpenes is still unclear. Oxidative squalene cyclase (OSC) can catalyze 2,3-Oxidosqualene into diverse products that serve as important precursors for triterpene synthesis. However, the function of the OSC gene in Chiridota sp. has not been elucidated. In this study, an OSC gene derived from the deep-sea holothurian Chiridota sp. was cloned and characterized functionally in a yeast system. The open reading frame of the OSC gene was 2086 bp, which encoded 695 amino acids. The Chiridota sp. OSC gene has a similarity of 66.89 % to the OSC of other holothurian species and 63.51 % to that of Acanthaster planci. The phylogenetic tree showed that the echinozoan OSCs clustered together, and then they formed a sister group to fungi and plant homologs. Chiridota sp. OSC catalyzed 2,3-Oxidosqualene into parkeol. Under high pressure, the relative enzymatic activity and stability of cyclase in Chiridota sp. was higher than that in the shallow-sea holothurian Stichopus horrens. The newly cloned OSC of Chiridota sp. provides key information for the interpretation of the saponin synthesis pathway in deep-sea holothurians.
WOS关键词BETA-AMYRIN SYNTHASE ; HIGH-PRESSURE ; TRITERPENE GLYCOSIDES ; OXIDOSQUALENE CYCLASE ; BIOSYNTHESIS ; SAPONINS ; CYCLOARTENOL ; EXPRESSION ; LANOSTEROL
资助项目Major scientific and technological projects of Hainan Province[ZDKJ2021036] ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA22050303] ; National Key Research and Development Program of China[2016YFC0304905]
WOS研究方向Genetics & Heredity
语种英语
出版者ELSEVIER
WOS记录号WOS:001119752000001
资助机构Major scientific and technological projects of Hainan Province ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; National Key Research and Development Program of China
源URL[http://ir.idsse.ac.cn/handle/183446/10712]  
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
研究生部
通讯作者Zhang, Haibin
作者单位1.Chinese Acad Sci, Inst Deep sea Sci & Engn, Sanya 572000, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Ocean Univ China, Qingdao 266100, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Yanan,Liu, Helu,Zhou, Yang,et al. Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.[J]. GENE,2024,894:9.
APA Huang, Yanan,Liu, Helu,Zhou, Yang,Lu, Zaiqing,Pu, Yujin,&Zhang, Haibin.(2024).Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp..GENE,894,9.
MLA Huang, Yanan,et al."Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.".GENE 894(2024):9.

入库方式： OAI收割

来源：深海科学与工程研究所

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