Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.
文献类型：期刊论文
| 作者 | Wang, Kang1,2; Li, Wenjun1 ; Cui, Hongli1; Qin, Song1
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2023-10-01 |
| 卷号 | 24期号:20页码:11 |
| ISSN号 | 1661-6596 |
| 关键词 | c-di-GMP Arthrospira diguanylate cyclase phosphodiesterase riboswitch |
| DOI | 10.3390/ijms242015210 |
| 通讯作者 | Qin, Song(sqin@yic.ac.cn) |
| 英文摘要 | Cyclic di-GMP (c-di-GMP) is a second messenger of intracellular communication in bacterial species, which widely modulates diverse cellular processes. However, little is known about the c-di-GMP network in filamentous multicellular cyanobacteria. In this study, we preliminarily investigated the c-di-GMP turnover proteins in Arthrospira based on published protein data. Bioinformatics results indicate the presence of at least 149 potential turnover proteins in five Arthrospira subspecies. Some proteins are highly conserved in all tested Arthrospira, whereas others are specifically found only in certain subspecies. To further validate the protein catalytic activity, we constructed a riboswitch-based c-di-GMP expression assay system in Escherichia coli and confirmed that a GGDEF domain protein, Adc11, exhibits potential diguanylate cyclase activity. Moreover, we also evaluated a protein with a conserved HD-GYP domain, Ahd1, the expression of which significantly improved the swimming ability of E. coli. Enzyme-linked immunosorbent assay also showed that overexpression of Ahd1 reduced the intracellular concentration of c-di-GMP, which is presumed to exhibit phosphodiesterase activity. Notably, meta-analyses of transcriptomes suggest that Adc11 and Ahd1 are invariable. Overall, this work confirms the possible existence of a functional c-di-GMP network in Arthrospira, which will provide support for the revelation of the biological function of the c-di-GMP system in Arthrospira. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001099386200001 |
| 资助机构 | High-tech industrialization special project in cooperation between Jilin Province ; Chinese Academy of Sciences ; Major Science and Technology Innovation Project of Shandong Province ; Natural Science Foundation of Shandong Province |
| 源URL | [http://ir.yic.ac.cn/handle/133337/33237]  |
| 专题 | 中国科学院烟台海岸带研究所 |
| 通讯作者 | Qin, Song |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Kang,Li, Wenjun,Cui, Hongli,et al. Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(20):11. |
| APA | Wang, Kang,Li, Wenjun,Cui, Hongli,&Qin, Song.(2023).Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp..INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(20),11. |
| MLA | Wang, Kang,et al."Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.20(2023):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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