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Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

文献类型：期刊论文

作者Zhang, Weikang1,4; Lin, Yingwu3; Meng, Xiangmin4; Wang, Qiaoning2; Chen, Lingxin2; Xu, Jiakun1
刊名JOURNAL OF HAZARDOUS MATERIALS
出版日期2024-03-05
卷号465页码:10
ISSN号0304-3894
关键词Sulfonamides Myoglobin Biocatalysis Biodegradation Directed evolution
DOI10.1016/j.jhazmat.2023.133147
通讯作者Chen, Lingxin(lxchen@yic.ac.cn) ; Xu, Jiakun(xujk@ysfri.ac.cn)
英文摘要Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation
WOS关键词COMPOUND-I ; ANTIBIOTICS ; SULFAMETHOXAZOLE ; SULFONAMIDES ; KINETICS ; BIOTRANSFORMATION ; SULFAMETHAZINE ; TRANSFORMATION ; DEGRADATION ; ENVIRONMENT
WOS研究方向Engineering ; Environmental Sciences & Ecology
语种英语
WOS记录号WOS:001134426400001
资助机构National Key R & D Program of China ; National Natural Science Foundation of China ; Central Public-Interest Scientific Institution Basal Research Fund, CAFS
源URL[http://ir.yic.ac.cn/handle/133337/33117]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Chen, Lingxin; Xu, Jiakun
作者单位1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Lab Marine Drugs & Byprod Pilot Natl Lab Marine Sc, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
4.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,et al. Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,465:10.
APA Zhang, Weikang,Lin, Yingwu,Meng, Xiangmin,Wang, Qiaoning,Chen, Lingxin,&Xu, Jiakun.(2024).Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole.JOURNAL OF HAZARDOUS MATERIALS,465,10.
MLA Zhang, Weikang,et al."Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole".JOURNAL OF HAZARDOUS MATERIALS 465(2024):10.

入库方式： OAI收割

来源：烟台海岸带研究所

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