Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array
文献类型：期刊论文
| 作者 | Zhang, Han3,4; Mou, Junsong3,4; Ding, Jiawang1,2,4 ; Qin, Wei1,2,4
|
| 刊名 | SSRN
 |
| 出版日期 | 2023 |
| 关键词 | Antibiotics Cell death Peptides Potentiometers (electric measuring instruments) |
| ISSN号 | 15565068 |
| DOI | 10.2139/ssrn.4636337 |
| 英文摘要 | Phenotypic antimicrobial susceptibility testing that identifies the phenotypic feature differences of bacteria after incubation with antibiotics enables reliable antibiotics screening but is restricted by bacterial proliferation rate. Here, a magneto-controlled potentiometric sensors array was developed for rapid antibiotic screening based on direct detection of apoptotic bacteria. Phosphatidylserine (PS)-binding peptide serving as bioreceptor and signal transducer was immobilized on magnetic beads (MBs-peptide), and could selectively capture apoptotic bacteria killed by antibiotics. Apoptotic bacteria binding-induced charge density change of MBs-peptide resulted in a potential change on a magneto-controlled polymeric membrane potentiometric sensor. Based on the apoptotic bacteria detection, antimicrobial activities of micrograms per milliliter antibiotics could be evaluated within 1.5 h, which were hardly achieved by current methods. In addition, the antibacterial ability of different antibiotics could be evaluated simultaneously using a potentiometric sensors array. This approach enables sensitive, general, and time-saving antibiotic screening, and may open up a new path for antibiotic susceptibility testing. © 2023, The Authors. All rights reserved. |
| 语种 | 英语 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/34288]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_近岸生态与环境实验室 |
| 作者单位 | 1.Qingdao National Laboratory for Marine Science and Technology, Shandong, Qingdao; 266237, China; 2.Center for Ocean Mega-Science, Chinese Academy of Sciences, Shandong, Qingdao; 266071, China; 3.University of Chinese Academy of Sciences, Beijing; 100049, China 4.CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Shandong, Yantai; 264003, China; |
| 推荐引用方式 GB/T 7714 | Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array[J]. SSRN,2023. |
| APA | Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2023).Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array.SSRN. |
| MLA | Zhang, Han,et al."Rapid Antibiotic Screening Based on Bacteria Apoptosis Using Potentiometric Sensor Array".SSRN (2023). |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。