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Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families

文献类型：期刊论文

作者Sluchanko, Nikolai N.4; Maksimov, Eugene G.3; Slonimskiy, Yury B.4; Varfolomeeva, Larisa A.4; Bukhanko, Antonina Y.3; Egorkin, Nikita A.3,4; Tsoraev, Georgy, V4; Khrenova, Maria G.2,4; Ge, Baosheng1; Qin, Song5
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2024
卷号254页码:20
ISSN号0141-8130
关键词Photoprotection Phycobilisome fluorescence quenching Crystal structure Protein-protein interaction Absorption spectroscopy Photoactivity SEC-MALS
DOI10.1016/j.ijbiomac.2023.127874
通讯作者Sluchanko, Nikolai N.(nikolai.sluchanko@mail.ru) ; Qin, Song(sqin@yic.ac.cn)
英文摘要The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non -photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP, as well as with the rotation of the echinenone's beta-ionone ring in the CTD. Structural data also helped rationalize the observed differences in OCP/ FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering.
WOS关键词N-TERMINAL DOMAIN ; PHOTOPROTECTIVE MECHANISM ; CRYSTAL-STRUCTURE ; SIGNALING STATE ; RECOVERY ; IDENTIFICATION ; TRANSLOCATION ; FLUORESCENCE ; MUTANT ; COLOR
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001113594000001
资助机构Program of the Ministry of Science and Higher Education of the Russian Federation ; joint Russian Science Foundation/National Scientific Fund of China ; Program of the Ministry of Science and Higher Education of the Russian Federation ; Russian Science Foundation/National Scientific Fund of China
源URL[http://ir.yic.ac.cn/handle/133337/33162]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者Sluchanko, Nikolai N.; Qin, Song
作者单位1.China Univ Petr Huadong, Coll Chem & Chem Engn, Qingdao 266580, Peoples R China
2.Lomonosov Moscow State Univ, Chem Dept, Moscow 119991, Russia
3.Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia
4.AN Bach Inst Biochem, Fed Res Ctr Biotechnol Russian Acad Sci, AN Bach Inst Biochem, Moscow 119071, Russia
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Sluchanko, Nikolai N.,Maksimov, Eugene G.,Slonimskiy, Yury B.,et al. Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,254:20.
APA Sluchanko, Nikolai N..,Maksimov, Eugene G..,Slonimskiy, Yury B..,Varfolomeeva, Larisa A..,Bukhanko, Antonina Y..,...&Popov, Vladimir O..(2024).Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,254,20.
MLA Sluchanko, Nikolai N.,et al."Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 254(2024):20.

入库方式： OAI收割

来源：烟台海岸带研究所

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