中国明对虾BMP-7基因的克隆及表达模式分析
文献类型：期刊论文
| 作者 | 高娜1,2,3; 卢霞4 ; 闫允君2,3; 孔杰2,3; 孟宪红2,3; 李旭鹏2,3; 隋娟2,3; 陈宝龙2,3; 曹宝祥2,3; 高焕1
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| 刊名 | 水产科学
 |
| 出版日期 | 2023 |
| 卷号 | 42期号:3页码:347-356 |
| ISSN号 | 1003-1111 |
| 关键词 | 中国明对虾 BMP-7基因 克隆 表达模式 |
| 其他题名 | Cloning and Expression of FcBMP-7 Gene in Chinese Shrimp Fenneropenaeus chinensis |
| 文献子类 | 期刊论文 |
| 英文摘要 | In order to analyze the expression of bone morphogenetic protein-7 (BMP-7) gene,a member of transforming growth factor beta (TGF-beta) superfamily, in the muscle growth and development of Chinese shrimp Fenneropenaeus chinensis,the BMP-7 (FcBMP-7) was cloned by through Rapid Amplification of cDNA Ends (RACE) technology. The full-length cDNA of FcBMP-7 was 2003 bp, containing 466 bp of 5' untranslated region(UTR),295 bp of 3' UTR and 1242 bp of open reading frame(ORF),encoding 413 amino acids. Using real-time PCR technology to analyze the expression pattern of FcBMP-7 in the muscle tissues of Chinese shrimp larvae at five different developmental stages (gastrulation stage,nauplius stage, zoea stage,mysis stage,and postlarval stage). The results showed that the gene was expressed in all stages of larval development,with the minimal expression in the gastrula stage,the significant increase in expression level from the nauplius stage (P<0.05), and the maximal expression level in the mysis stage; indicating that FcBMP-7 was involved in the development of shrimp muscle. Expression pattern of FcBMP-7 in 8 tissues of two sizes of Chinese shrimp revealed that FcBMP-7 had extensive tissue expression. There were significantly different in the expression levels in most tissues between the two groups (P<0.05),especially in the heart,intestine,muscle,periopod and pleopod (P<0.01). The findings suggested that FcBMP-7 gene played a certain role in the muscle growth and development of Chinese shrimp,which would lay a theoretical foundation for further analysis of the molecular mechanism of muscle development in Chinese shrimp. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:7471157 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/34229]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点试验室,江苏连云港222005; 2.中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛266071; 3.青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266071; 4.中国科学院烟台海岸带研究所,沿海生物资源高效利用研究开发中心,山东烟台264003 |
| 推荐引用方式 GB/T 7714 | 高娜,卢霞,闫允君,等. 中国明对虾BMP-7基因的克隆及表达模式分析[J]. 水产科学,2023,42(3):347-356. |
| APA | 高娜.,卢霞.,闫允君.,孔杰.,孟宪红.,...&栾生.(2023).中国明对虾BMP-7基因的克隆及表达模式分析.水产科学,42(3),347-356. |
| MLA | 高娜,et al."中国明对虾BMP-7基因的克隆及表达模式分析".水产科学 42.3(2023):347-356. |
入库方式： OAI收割
来源：烟台海岸带研究所
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