UV-B对雨生红球藻生长及虾青素积累的影响
文献类型：期刊论文
| 作者 | 许文鑫1,3; 朱琴1,3; 朱梅1,3; 季春丽1,3; 张春辉1,3; 秦松2 ; 李润植1,3; 崔红利1,3
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| 刊名 | 水生生物学报
 |
| 出版日期 | 2021 |
| 卷号 | 45期号:6页码:1281-1290 |
| 关键词 | 雨生红球藻 紫外光B波段(UV-B) 紫外光受体UVR8 虾青素合成 光信号通路 |
| ISSN号 | 1000-3207 |
| 其他题名 | ULTRAVIOLET-B RADIATION ENHANCES THE GROWTH AND ASTAXANTHIN PRODUCTION IN HAEMATOCOCCUS PLUVIALIS |
| 文献子类 | 期刊论文 |
| 英文摘要 | Haematococcus pluvialis is recognized as the ideal astaxanthin producer since high levels of astaxanthin can be accumulated in its cells under stress conditions. Although appropriate amount of ultraviolet-B (UV-B) can effectively induce astaxanthin biosynthesis, it remains to be elucidated whether the specific light signal perception and transduction mechanisms regulate astaxanthin accumulation in this alga. The present study was conducted to investigate the effects of different intensity of UV-B on the growth, photosynthesis and production of astaxanthin of H. pluvialis under six cultivation conditions (100-500 lux of UV-B was supplemented under LED light culture conditions). A series of physiological and biochemical parameters were examined under these six UV-B treatments designed as CK, U100, U200, U300, U400 and U500, respectively. The results revealed that UV-B radiation decreased the cell density, maximal quantum yield of PSⅡ (F_v/F_m), non-photochemical quenching parameter (NPQ) and chlorophyll (Chl. a and Chl. b) content in H. pluvialis. On the contrary, the astaxanthin content were enhanced with the increase of UV-B radiation intensity (100-400 lx). Compared with the control algal cells without UV-B treatment, astaxanthin content increased by 35.68% and 56.23% in the algal cells treated by 36h and 72h of high-intensity UV-B radiation (U400), with the level up to 5.82 and 7.06 mg/L. Expression analysis by qRT-PCR demonstrated that both 200 and 400 lx UV-B treatments significantly upregulated transcriptional expression of the four carotenoid biosynthesis-related genes (IPI, PSY, BCH and BKT) in H. pluvialis despite their expression profiles were different. UV-B radiation also upregulated the expression of UVR8 and the downstream genes COP1, SPA1, HYH and HY5 which are the core elements in UV-B signal transduction pathway. These findings indicated that the UV-B light signal transduction pathway may function importantly in the regulation of astaxanthin biosynthesis pathway, providing a scientific basis for optimizing culture system to increase astaxanthin accumulation in H. pluvialis by UV-B auxiliary light source. The present data also lay the foundation for further understanding the transcriptional regulation mechanisms of astaxanthin accumulation in this special agal under stress conditions, particularly in response to UV-B radiation. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:7127636 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/34236]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.山西农业大学分子农业与生物能源研究所,太谷030801; 2.中国科学院烟台海岸带研究所,烟台264003 3.山西农业大学农学院,太谷030801; |
| 推荐引用方式 GB/T 7714 | 许文鑫,朱琴,朱梅,等. UV-B对雨生红球藻生长及虾青素积累的影响[J]. 水生生物学报,2021,45(6):1281-1290. |
| APA | 许文鑫.,朱琴.,朱梅.,季春丽.,张春辉.,...&崔红利.(2021).UV-B对雨生红球藻生长及虾青素积累的影响.水生生物学报,45(6),1281-1290. |
| MLA | 许文鑫,et al."UV-B对雨生红球藻生长及虾青素积累的影响".水生生物学报 45.6(2021):1281-1290. |
入库方式： OAI收割
来源：烟台海岸带研究所
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