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Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells

文献类型：期刊论文

作者Lin, Jia-Ling7,8; Chen, Longxian8; Wu, Wen-Kai8; Guo, Xiao-Xiang8; Yu, Cheng-Hui6; Xu, Min7; Nie, Gui-Bin8; Dun, Jun-ling5; Li, Yan3,4; Xu, Baofu
刊名MOLECULAR PLANT
出版日期2023-12-04
卷号16期号:12页码:1990-2003
ISSN号1674-2052
DOI10.1016/j.molp.2023.10.008
通讯作者Gao, Wei(gaowei021@163.com) ; Huang, Jin-Quan(huangjinquan@cemps.ac.cn)
英文摘要Plants can synthesize a wide range of terpenoids in response to various environmental cues. However, the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive. In this study, we employed single-cell RNA sequencing to comprehensively characterize the tran-scriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cell-specific terpenoid production. We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes (such as f3-caryophyllene and f3-myrcene) and non-volatile gossypol-type terpenoids in secretory glandular cells. Moreover, two novel transcription factors, namely GoHSFA4a and GoNAC42, are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes. Both transcription factors could directly regulate the expression of terpenoid biosyn-thetic genes in secretory glandular cells in response to developmental and environmental stimuli. For convenient retrieval of the single-cell RNA sequencing data generated in this study, we developed a user-friendly web server . Our findings not only offer valuable insights into the precise regulation of terpe-noid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.
WOS关键词VOLATILE EMISSIONS ; STOMATAL LINEAGE ; EXPRESSION ; DIFFERENTIATION ; SYNTHASES ; TOOL
资助项目Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences[XDB27020207] ; Young Elite Scientists Sponsorship Program by CAST[2019QNRC001] ; Yunnan Revitalization Talent Support Pro-gram Top TeamProject ; National Natural Science Foundation of China[202305AT350001] ; National Natural Science Foundation of China[GMLM2023] ; [32172041]
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:001131718700001
出版者CELL PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/308371]  
专题新药研究国家重点实验室
通讯作者Gao, Wei; Huang, Jin-Quan
作者单位1.Henan Univ, Natl Key Lab Cotton Biobreeding & Integrated Utili, Henan 475004, Peoples R China
2.Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai 201602, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
5.Shimadzu China Co Ltd, Analyt Applicat Ctr, Shanghai 200233, Peoples R China
6.Chongqing Technol & Business Univ, Natl Res Base Intelligent Mfg Serv, Chongqing Key Lab Micronano Syst & Intelligent Tra, Chongqing 400067, Peoples R China
7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
8.Univ CAS, Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci,State Key Lab Pla, Shanghai 200032, Peoples R China
推荐引用方式
GB/T 7714		 Lin, Jia-Ling,Chen, Longxian,Wu, Wen-Kai,et al. Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells[J]. MOLECULAR PLANT,2023,16(12):1990-2003.
APA Lin, Jia-Ling.,Chen, Longxian.,Wu, Wen-Kai.,Guo, Xiao-Xiang.,Yu, Cheng-Hui.,...&Huang, Jin-Quan.(2023).Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells.MOLECULAR PLANT,16(12),1990-2003.
MLA Lin, Jia-Ling,et al."Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells".MOLECULAR PLANT 16.12(2023):1990-2003.

入库方式： OAI收割

来源：上海药物研究所

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