DNA Aptamer Binding Octapeptide Repeat Region of Cellular Prion Protein
文献类型：期刊论文
| 作者 | Sheng, Jing1,4; Zhang, Nan4; Long, Zhenhao1,4; Zhang, Xiangru1,4; Zu, Shuang2,3; Liu, Xiangjun1,4; Shangguan, Dihua1,3,4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2023-12-04 |
| 卷号 | 95期号:50页码:18595-18602 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.3c04557 |
| 通讯作者 | Shangguan, Dihua(sgdh@iccas.ac.cn) |
| 英文摘要 | Cellular prion protein (PrP (c)) is highly expressed in a variety of tumor cells and plays a crucial role in neurodegenerative diseases. Its N-terminal domain contains a conserved octapeptide (PHGGGWGQ) repeat sequence. The number of repeats has been correlated with the species as well as the development of associated diseases. Herein, PrP (c) was identified to be the molecular target of a high-affinity DNA aptamer HA5-68 obtained by cell-SELEX. Aptamer HA5-68 was further optimized to two short sequences (HA5-40-1 and HA5-40-2), and its binding site to PrP (c) was identified to be located in the loop-stem-loop region of the head of its secondary structure. HA5 series aptamers were demonstrated to bind the octapeptide repeat region of PrP (c), as well as the synthesized peptides containing different numbers of octapeptide repeats. The PrP (c) expression on 42 cell lines was measured by using aptamer HA5-68 as a molecular probe. The clear understanding of the molecular structure and binding mechanism of this set of aptamers will provide information for the design of diagnostic methods and therapeutic drugs targeting PrP (c). |
| WOS关键词 | CREUTZFELDT-JAKOB-DISEASE ; IN-VITRO SELECTION ; CANCER-CELLS |
| 资助项目 | Beijing Municipal Natural Science Foundation[22174149] ; National Natural Science Foundation of China[2022YFA1304500] ; National Key Research and Development Program of China[7232263] ; Beijing Natural Science Foundation |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:001129609800001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/308377]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Chem Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Mol Med, Hangzhou 310013, Peoples R China 4.Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sheng, Jing,Zhang, Nan,Long, Zhenhao,et al. DNA Aptamer Binding Octapeptide Repeat Region of Cellular Prion Protein[J]. ANALYTICAL CHEMISTRY,2023,95(50):18595-18602. |
| APA | Sheng, Jing.,Zhang, Nan.,Long, Zhenhao.,Zhang, Xiangru.,Zu, Shuang.,...&Shangguan, Dihua.(2023).DNA Aptamer Binding Octapeptide Repeat Region of Cellular Prion Protein.ANALYTICAL CHEMISTRY,95(50),18595-18602. |
| MLA | Sheng, Jing,et al."DNA Aptamer Binding Octapeptide Repeat Region of Cellular Prion Protein".ANALYTICAL CHEMISTRY 95.50(2023):18595-18602. |
入库方式： OAI收割
来源：上海药物研究所
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