Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches
文献类型：期刊论文
| 作者 | Xu, Hui; Li, Zhen3,4; Jiang, Peng-Fei; Zhao, Li5; Qu, Chang; Van de Peer, Yves3,4,6; Liu, Yan-Jing; Zeng, Qing-Yin |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2022 |
| 卷号 | 110期号:1页码:129-146 |
| 关键词 | active site motif gene family functional divergence glutaredoxin enzymatic specificity Populus trichocarpa |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.15660 |
| 文献子类 | Article |
| 英文摘要 | Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | ARABIDOPSIS CHLOROPLASTIC GLUTAREDOXIN ; GENE FAMILY ; SUBCELLULAR RELOCALIZATION ; FUNCTIONAL DIVERGENCE ; CATALYTIC-PROPERTIES ; YEAST GLUTAREDOXINS ; ESCHERICHIA-COLI ; SUPERGENE FAMILY ; DUPLICATED GENES ; REDOX REGULATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000759059500001 |
| 出版者 | WILEY |
| 资助机构 | National Science Foundation of China [31822011, 31425006] ; Chinese Academy of Forestry [CAFYBB2018ZX001] ; Special Research Fund of Ghent University [BOFPDO2018001701] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28491]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Univ Pretoria, Dept Biochem Genet & Microbiol, ZA-0028 Pretoria, South Africa 2.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium 5.VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium 6.Umea Univ, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden |
| 推荐引用方式 GB/T 7714 | Xu, Hui,Li, Zhen,Jiang, Peng-Fei,et al. Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches[J]. PLANT JOURNAL,2022,110(1):129-146. |
| APA | Xu, Hui.,Li, Zhen.,Jiang, Peng-Fei.,Zhao, Li.,Qu, Chang.,...&Zeng, Qing-Yin.(2022).Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches.PLANT JOURNAL,110(1),129-146. |
| MLA | Xu, Hui,et al."Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches".PLANT JOURNAL 110.1(2022):129-146. |
入库方式： OAI收割
来源：植物研究所
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