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Anti-Inflammatory Functions of Methanol Extract from Malus baccata (L.) Borkh. Leaves and Shoots by Targeting the NF-κB Pathway

文献类型：期刊论文

作者Song, Chaoran; Chen, Hongxi; Kim, Soo Ah5; Lee, Jong Sub5; Choung, Eui Su5; Zhang, Zhiyun1; Kim, Soo-Yong; Kim, Jong-Hoon; Cho, Jae Youl
刊名PLANTS-BASEL
出版日期2022
卷号11期号:5
关键词Malus baccata (L.) Borkh. anti-inflammatory NF-kappa B
DOI10.3390/plants11050646
文献子类Article
英文摘要Malus baccata (L.) Borkh. is a widely used medical plant in Asia. Since the anti-inflammatory mechanism of this plant is not fully understood, the aim of this study was to explore the anti-inflammatory function and mechanism of Malus baccata (L.) Borkh. methanol extract (Mb-ME). For in vitro experiments, nitric oxide production assay, PCR, overexpression strategy, immunoblotting, luciferase reporter assay, and immunoprecipitation were employed to explore the molecular mechanism and the target proteins of Mb-ME. For in vivo experiments, an HCl/EtOH-induced gastritis mouse model was used to confirm the anti-inflammatory function. Mb-ME showed a strong ability to inhibit the production of nitric oxide and the expression of inflammatory genes. Mb-ME decreased NF-kappa B luciferase activity mediated by MyD88 and TRIF. Moreover, Mb-ME blocked the activation of Src, Syk, p85, Akt, p50, p60, IKK alpha/beta, and I kappa B alpha in LPS-induced RAW264.7 cells. Overexpression and immunoprecipitation analyses suggested Syk and Src as the target enzymes of Mb-ME. In vitro results showed that Mb-ME could alleviate gastritis and relieve the protein expression of p-Src, p-Syk, and COX-2, as well as the gene expression of COX-2 and TNF-alpha. In summary, this study implied that Mb-ME performs an anti-inflammatory role by suppressing Syk and Src in the NF-kappa B signaling pathway, both in vivo and in vitro.
学科主题Plant Sciences
电子版国际标准刊号2223-7747
出版地BASEL
WOS关键词PANAX-GINSENG ; INFLAMMATORY RESPONSES ; MACROPHAGE ACTIVATION ; APPLE FRUITS ; GINSENOSIDES ; LPS ; SRC ; INHIBITION ; SYK
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者MDPI
WOS记录号WOS:000784191900001
资助机构Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education, Korea [2017R1A6A1A03015642] ; KRF
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28774]  
专题系统与进化植物学国家重点实验室
作者单位1.DanjoungBio Co Ltd, Wonju 26303, South Korea
2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
3.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea
4.Chonbuk Natl Univ, Coll Vet Med, Iksan 54596, South Korea
5.Biomed Inst Convergence SKKU BICS, Dept Integrat Biotechnol, Suwon 16419, South Korea
推荐引用方式
GB/T 7714		 Song, Chaoran,Chen, Hongxi,Kim, Soo Ah,et al. Anti-Inflammatory Functions of Methanol Extract from Malus baccata (L.) Borkh. Leaves and Shoots by Targeting the NF-κB Pathway[J]. PLANTS-BASEL,2022,11(5).
APA Song, Chaoran.,Chen, Hongxi.,Kim, Soo Ah.,Lee, Jong Sub.,Choung, Eui Su.,...&Cho, Jae Youl.(2022).Anti-Inflammatory Functions of Methanol Extract from Malus baccata (L.) Borkh. Leaves and Shoots by Targeting the NF-κB Pathway.PLANTS-BASEL,11(5).
MLA Song, Chaoran,et al."Anti-Inflammatory Functions of Methanol Extract from Malus baccata (L.) Borkh. Leaves and Shoots by Targeting the NF-κB Pathway".PLANTS-BASEL 11.5(2022).

入库方式： OAI收割

来源：植物研究所

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