Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons
文献类型：期刊论文
| 作者 | Fozard, John A.; Yu, Man; Bezodis, William; Cheng, Jie1,3; Spooner, Jamie; Mansfield, Catherine; Chan, Jordi; Coen, Enrico |
| 刊名 | CURRENT BIOLOGY
 |
| 出版日期 | 2022 |
| 卷号 | 32期号:22页码:4967-4974 |
| ISSN号 | 0960-9822 |
| DOI | 10.1016/j.cub.2022.09.049 |
| 文献子类 | Article |
| 英文摘要 | Many plant cells exhibit polarity, revealed by asymmetric localization of specific proteins within each cell.1-6 Polarity is typically coordinated between cells across a tissue, raising the question of how coordination is achieved. One hypothesis is that mechanical stresses provide cues.7 This idea gains support from experiments in which cotyledons were mechanically stretched transversely to their midline.8 These previously published re-sults showed that without applied tension, the stomatal lineage cell polarity marker, BREVIS RADIX-LIKE 2 (BRXL2), exhibited no significant excess in the transverse orientation. By contrast, 7 h after stretching, BRXL2 polarity distribution exhibited transverse excess, aligned with the stretch direction. These stretching ex-periments involved statistical comparisons between snapshots of stretched and unstretched cotyledons, with different specimens being imaged in each case.8 Here, we image the same cotyledon before and after stretching and find no evidence for reorientation of polarity. Instead, statistical analysis shows that cotyledons contain a pre-existing transverse excess in BRXL2 polarity orientation that is not significantly modified by applied tension. The transverse excess reflects BRLX2 being preferentially localized toward the medial side of the cell, nearer to the cotyledon midline, creating a weak medial bias. A second polarity marker, BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), also exhibits weak medial bias in stomatal lineages, whereas ectopic expres-sion of BASL in non-stomatal cells exhibits strong proximal bias, as previously observed in rosette leaves. This proximal bias is also unperturbed by applied tension. Our findings therefore show that cotyledons contain two near-orthogonal coordinated biases in planar polarity: mediolateral and proximodistal. |
| 学科主题 | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 电子版国际标准刊号 | 1879-0445 |
| 出版地 | CAMBRIDGE |
| WOS关键词 | ASYMMETRIC CELL-DIVISION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000901528800011 |
| 资助机构 | UK Biotechnology and Biological Sciences Research Council ; [BB/W007924/1] ; [BBS/E/J/000PR9787] ; [BBS/E/J/00000152] ; [BB/L008920/1] ; [BBS/E/J/000PR9789] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28824]  |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, England |
| 推荐引用方式 GB/T 7714 | Fozard, John A.,Yu, Man,Bezodis, William,et al. Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons[J]. CURRENT BIOLOGY,2022,32(22):4967-4974. |
| APA | Fozard, John A..,Yu, Man.,Bezodis, William.,Cheng, Jie.,Spooner, Jamie.,...&Coen, Enrico.(2022).Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons.CURRENT BIOLOGY,32(22),4967-4974. |
| MLA | Fozard, John A.,et al."Localization of stomatal lineage proteins reveals contrasting planar polarity patterns in Arabidopsis cotyledons".CURRENT BIOLOGY 32.22(2022):4967-4974. |
入库方式： OAI收割
来源：植物研究所
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