Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping
文献类型：期刊论文
| 作者 | Wang, Lan1,5; Liu, Yang1,5; Zhao, Haiyan2; Zheng, Yuebin1,3; Bai, Feng1,5; Deng, Sicheng; Chen, Zhixiong1,5; Wu, Jinwen1,5; Liu, Xiangdong1,5 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2022 |
| 卷号 | 13 |
| ISSN号 | 1664-462X |
| 关键词 | wild rice grain length BSA fine mapping gene cloning |
| DOI | 10.3389/fpls.2022.921029 |
| 文献子类 | Article |
| 英文摘要 | Grain length (GL) directly affects the yield and quality of rice. Very few cloned GL-related genes are applied in production because their yield-increasing effects are not obvious, and the overall regulatory networks underlying the associated processes remain poorly understood. DNA samples from two bulk DNA pools (L-pool and S-pool) and their parents (KJ01 and Huaye 3) were subjected to high-throughput sequencing. Using bulked segregant analysis (BSA), qGL3.5 was mapped to a 0.34-Mb hotspot region on chromosome 3 that contains 37 genes related to various traits. Then, qGL3.5 was mapped to the genomic interval between the flanking markers M2 and M3 using 2786 BC4F2 individuals. Because the region from B5 to B6 was not the associated region under BSA-seq analysis, qGL3.5 was narrowed down to the interval between B6 and M3, which spanned 24.0-kb. Of all 37 genes with non-synonymous single-nucleotide polymorphisms (SNPs) between KJ01 and Huaye 3 based on BSA-seq analysis, only one complete annotated gene, ORF18 (Gene ID: LOC_Os03g42790.1) was found. ORF18 encodes an IBR-RING zinc-finger-related protein, with one really interesting new gene (RING) and two in between ring finger (IBR) domains. The knockout of ORF18 derived from Huaye 3 using clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) editing technology increased the GL of the mutant by approximately 2.2 mm. The novel locus qGL3.5 negatively regulated GL by promoting elongation of the longitudinal cell of the grain outer glume. These results provide a new genetic resource for rice grain shape breeding and a starting point for the functional characterization of the wild rice GL gene. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | QUANTITATIVE TRAIT LOCI ; GENETIC-ANALYSIS ; SIZE ; NUMBER ; YIELD ; SEQ ; DNA |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000817988900001 |
| 资助机构 | Laboratory of Lingnan Modern Agriculture Project [NT2021001] ; Guangdong Provincial Natural Science Foundation Program [2019A1515011826] ; Guangdong Provincial Special Projects in Key Field of Rural Revitalization [2020ZDZX1038] ; University Students' Innovation Project of South China Agricultural University [S202010564128] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28883]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China 2.South China Agr Univ, Coll Agr, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou, Peoples R China 3.Songgang Middle Sch, Qingyuan, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 5.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lan,Liu, Yang,Zhao, Haiyan,et al. Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping[J]. FRONTIERS IN PLANT SCIENCE,2022,13. |
| APA | Wang, Lan.,Liu, Yang.,Zhao, Haiyan.,Zheng, Yuebin.,Bai, Feng.,...&Liu, Xiangdong.(2022).Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping.FRONTIERS IN PLANT SCIENCE,13. |
| MLA | Wang, Lan,et al."Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping".FRONTIERS IN PLANT SCIENCE 13(2022). |
入库方式： OAI收割
来源：植物研究所
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