Seed viability testing for research and conservation of epiphytic and terrestrial orchids
文献类型：期刊论文
| 作者 | Pradhan, Namrata2,4; Fan, Xuli6; Martini, Francesco2,3; Chen, Huayang7; Liu, Hong5; Gao, Jiangyun6; Goodale, Uromi Manage2,4 |
| 刊名 | BOTANICAL STUDIES
 |
| 出版日期 | 2022 |
| 卷号 | 63期号:1 |
| 关键词 | Plant conservation Seed banking Seed viability test Orchid seeds Epiphytic orchids Terrestrial orchids Lifeform Seed sterilization |
| DOI | 10.1186/s40529-022-00333-0 |
| 文献子类 | Article |
| 英文摘要 | Background Seed viability testing is essential in plant conservation and research. Seed viability testing determines the success of ex-situ conservation efforts, such as seed banking but commonly testing protocols of orchids lack consistency and accuracy, therefore, there is a need to select an appropriate and reliable viability test, especially when conducting comparative studies. Here, we evaluated the suitability of three seed viability tests, Evans blue test (EB), Fluorescein diacetate test (FDA) and Tetrazolium test (TTC), with and without sterilization, on seeds of 20 orchid species, which included five epiphytes and fifteen terrestrials, using both fresh seeds and seeds stored at - 18 oC for 6 to 8 years. Results We found that sterilization and lifeform of seeds affected seed viability across all tests but the storage time was not an influential factor. Sterilization negatively affected seed viability under EB and FDA test conditions but increased the detection of viable seeds in the TTC test in both epiphytic and terrestrial species. The EB test, when administered without sterilization provided the highest viability results. Being non-enzymatic unlike TTC and FDA tests, as expected, the EB test was the most reliable with similar results between sterilized and not sterilized seeds for most epiphytic and terrestrial species as well as when compared between groups. Conclusions The lifeform of the species and seed sterilization prior to testing are important influential factors in orchid seed viability testing. Since EB test was found to be reliable we recommend the EB test for seed viability assessment in orchids rather than the less reliable but commonly used TTC test, or the FDA test, which require more expensive and sophisticated instrumentation. Since storage time was not an influential factor in orchid seed viability testing, the recommendations of this study can be used for both fresh as well as long-term stored orchid seeds. This is helpful for research and especially for conservation measures such as seed banking. However, due to the species specificity of the bio-physiology of orchids, we call for comprehensive viability test assessment in the hyper diverse orchid family to be extended to a greater number of species to facilitate efficient conservation and research. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1999-3110 |
| 出版地 | NEW YORK |
| WOS关键词 | IN-VITRO GERMINATION ; ENCAPSULATION-DEHYDRATION ; ASYMBIOTIC GERMINATION ; TETRAZOLIUM ; CRYOPRESERVATION ; PROTOCORM ; CELLS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000753860500001 |
| 资助机构 | Guangxi Overseas High-level Talent Hundred People Program ; Lennox Boyd Trust Bursary Award ; Bagui Scholarship [C33600992001] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29037]  |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Natl Dong Hwa Univ, Ctr Interdisciplinary Res Ecol & Sustainabil, Hualien 97401, Taiwan 2.Guangxi Univ, Coll Forestry, Guangxi Key Lab Forest Ecol & Conservat, Daxuedonglu 100, Nanning 530004, Guangxi, Peoples R China 3.Florida Int Univ, Dept Earth & Environm, Int Ctr Trop Bot, 11200 SW 8th St, Miami, FL 33199 USA 4.Guangxi Univ, Coll Forestry, State Key Lab Conservat & Utilizat Subtrop Agrobi, Daxuedonglu 100, Nanning 530004, Guangxi, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 6.Species Survival Commiss, Seed Conservat Specialist Grp, Int Union Conservat Nat IUCN, CH-281196 Gland, Switzerland 7.Yunnan Univ, Lab Ecol & Evolutionary Biol, Chenggong Campus, Kunming 650504, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pradhan, Namrata,Fan, Xuli,Martini, Francesco,et al. Seed viability testing for research and conservation of epiphytic and terrestrial orchids[J]. BOTANICAL STUDIES,2022,63(1). |
| APA | Pradhan, Namrata.,Fan, Xuli.,Martini, Francesco.,Chen, Huayang.,Liu, Hong.,...&Goodale, Uromi Manage.(2022).Seed viability testing for research and conservation of epiphytic and terrestrial orchids.BOTANICAL STUDIES,63(1). |
| MLA | Pradhan, Namrata,et al."Seed viability testing for research and conservation of epiphytic and terrestrial orchids".BOTANICAL STUDIES 63.1(2022). |
入库方式： OAI收割
来源：植物研究所
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