MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple
文献类型：期刊论文
| 作者 | Dong, Haiqiang; Zheng, Qingbo2; Zhou, Yufei; Zhou, Yuwen; Bao, Zeyang; Lan, Qingqing; Li, Xu1 |
| 刊名 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2022 |
| 卷号 | 186页码:11-18 |
| 关键词 | Apple Adventitious shoot Regeneration MdWOX4-2 MdLBD41 |
| ISSN号 | 0981-9428 |
| DOI | 10.1016/j.plaphy.2022.06.026 |
| 文献子类 | Article |
| 英文摘要 | Apple (Malus domestica Borkh.) is not only an important fruit crop distributed worldwide, but also a common model plant. However, the lack of efficient genetic transformation procedures for apples limits the in-depth studies of their gene functions. Although leaf-regenerated adventitious shoots (LRAS) are a prerequisite for successful genetic transformation of apple, little is known about the underlying molecular mechanism of LRAS. Here, we identified the WUSCHEL-related homeobox (WOX) transcription factor in apple, MdWOX4-2, which was a transcriptional activator. Gene expression as well as morphological and histological observations revealed that MdWOX4-2 is involved in the development of LRAS. Overexpression of MdWOX4-2 conferred higher regenerative capacity in transgenic tobacco (Nicotiana tabacum) as compared to the wild type (WT). The com-bined results of the yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), dual luciferase assays, and transient transactivation assay, revealed that MdWOX4-2 directly bound to and activated the MdLBD41 promoter. Moreover, transgenic experiments further demonstrated that MdLBD41 could significantly enhance the formation of adventitious shoot in transgenic tobacco. Collectively, our findings demonstrate that MdWOX4-2 is important for regulating the LRAS development by activating MdLBD41. |
| 学科主题 | Plant Sciences |
| 出版地 | ISSY-LES-MOULINEAUX |
| 电子版国际标准刊号 | 1873-2690 |
| WOS关键词 | LATERAL ROOT-FORMATION ; WUSCHEL-RELATED HOMEOBOX4 ; CELL-PROLIFERATION ; GENE ; REGENERATION ; LEAF ; WOX4 ; DOWNSTREAM ; ACTIVATION ; REPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000830070200002 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | Zhejiang Provincial Top Discipline of Biological Engineering [(Level A) (ZS2021003), KF2022008] ; China Postdoctoral Science Foundation [2022M713004] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28466]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Zhejiang, Peoples R China 2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 10093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Haiqiang,Zheng, Qingbo,Zhou, Yufei,et al. MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2022,186:11-18. |
| APA | Dong, Haiqiang.,Zheng, Qingbo.,Zhou, Yufei.,Zhou, Yuwen.,Bao, Zeyang.,...&Li, Xu.(2022).MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple.PLANT PHYSIOLOGY AND BIOCHEMISTRY,186,11-18. |
| MLA | Dong, Haiqiang,et al."MdWOX4-2 modulated MdLBD41 functioning in adventitious shoot of apple".PLANT PHYSIOLOGY AND BIOCHEMISTRY 186(2022):11-18. |
入库方式： OAI收割
来源：植物研究所
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