Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants
文献类型：期刊论文
| 作者 | Wang, Jing1,5; Guo, Yanhong1; Yin, Xue1; Wang, Xiaoning4; Qi, Xiaoquan3 ; Xue, Zheyong1
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| 刊名 | CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
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| 出版日期 | 2022 |
| 卷号 | 57期号:2页码:113-132 |
| 关键词 | Triterpene oxidosqualene cyclase functional divergence catalytic mechanism biosynthesis |
| ISSN号 | 1040-9238 |
| DOI | 10.1080/10409238.2021.1979458 |
| 文献子类 | Review |
| 英文摘要 | Triterpenoids are one of the largest groups of secondary metabolites and exhibit diverse structures, which are derived from C30 skeletons that are biosynthesized via the isoprenoid pathway by cyclization of 2,3-oxidosqualene. Triterpenoids have a wide range of biological activities, and are used in functional foods, drugs, and as industrial materials. Due to the low content levels in their native plants and limited feasibility and efficiency of chemical synthesis, heterologous biosynthesis of triterpenoids is the most promising strategy. Herein, we classified 121 triterpene alcohols/ketones according to their conformation and ring numbers, among which 51 skeletons have been experimentally characterized as the products of oxidosqualene cyclases (OSCs). Interestingly, 24 skeletons that have not been reported from nature source were generated by OSCs in heterologous expression. Comprehensive evolutionary analysis of the identified 152 OSCs from 75 species in 25 plant orders show that several pentacyclic triterpene synthases repeatedly originated in multiple plant lineages. Comparative analysis of OSC catalytic reaction revealed that stabilization of intermediate cations, steric hindrance, and conformation of active center amino acid residues are primary factors affecting triterpene formation. Optimization of OSC could be achieved by changing of side-chain orientations of key residues. Recently, methods, such as rationally design of pathways, regulation of metabolic flow, compartmentalization engineering, etc., were introduced in improving chassis for the biosynthesis of triterpenoids. We expect that extensive study of natural variation of large number of OSCs and catalytical mechanism will provide basis for production of high level of triterpenoids by application of synthetic biology strategies. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | ABINGDON |
| 电子版国际标准刊号 | 1549-7798 |
| WOS关键词 | BETA-AMYRIN SYNTHASE ; OXIDOSQUALENE-LANOSTEROL CYCLASE ; ENCODING CYCLOARTENOL SYNTHASE ; CELL-SUSPENSION CULTURES ; SACCHAROMYCES-CEREVISIAE ; MOLECULAR-CLONING ; FUNCTIONAL EXPRESSION ; LUPEOL SYNTHASE ; STERIC BULK ; CATALYTIC ACTION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000702951600001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | National Natural Science Foundation of China including Major International (Regional) Joint Research Project and General Project [NSFC] [31920103003, 31770332, 31970314] ; Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28477]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Northeast Forestry Univ, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 3.Shandong Univ, Sch Pharmaceut Sci, Minist Educ, Dept Nat Prod Chem,Key Lab Chem Biol, Jinan, Peoples R China 4.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing, Peoples R China 5.Northeast Forestry Univ, Heilongjiang Key Lab Plant Bioact Subst Biosynthe, Harbin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jing,Guo, Yanhong,Yin, Xue,et al. Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants[J]. CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY,2022,57(2):113-132. |
| APA | Wang, Jing,Guo, Yanhong,Yin, Xue,Wang, Xiaoning,Qi, Xiaoquan,&Xue, Zheyong.(2022).Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants.CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY,57(2),113-132. |
| MLA | Wang, Jing,et al."Diverse triterpene skeletons are derived from the expansion and divergent evolution of 2,3-oxidosqualene cyclases in plants".CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY 57.2(2022):113-132. |
入库方式： OAI收割
来源：植物研究所
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