Lipid-Enhanced Photoprotection of LHCII in Membrane Nanodisc by Reducing Chlorophyll Triplet Production
文献类型：期刊论文
| 作者 | Yamano, Nami; Wang, Peng; Dong, Feng-Qin; Zhang, Jian-Ping |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2022 |
| 卷号 | 126期号:14页码:2669-2676 |
| ISSN号 | 1520-6106 |
| DOI | 10.1021/acs.jpcb.1c10557 |
| 文献子类 | Article |
| 英文摘要 | Carotenoid (Car) quenching chlorophyll triplet state ((3)Chl a*), an unwanted photosensitizer yielding harmful reactive oxygen species, is crucial for the survival of oxygenic photosynthetic organisms. For the major light-harvesting complex of photosystem II (LHCII) in isolated form, (3)Chl a* is deactivated via sub-nanosecond CM-to-Car triplet excitation energy transfer by lutein in the central domain of LHCII; however, the mechanistic difference from LHCII in vivo remains to be explored. To investigate the intrinsic Carphotoprotection properties of LHCII in a bio-mimicking circumstance, we reconstituted trimeric spinach LHCII into the discoidal membrane of nanosize made from L-alpha-phosphatidylcholine and examined the triplet excited dynamics. Time-resolved optical absorption combined with circular dichroism spectroscopies revealed that, with reference to LHCII in buffer, LHCII in the membrane nanodisc shows appreciable conformational variation in the neoxanthin and the Lut621 domains and in the CM a-terminal cluster owing to the lipid-protein interactions, which, in turn, alters the triplet population of Lut620 and Lut621 and their partition. Importantly, the unquenched (3)Chl a* population in the complex was reduced by 60%, indicating that LHCII in the membrane adopts a conformation that is optimized for the alleviation of photoinhibition. |
| 学科主题 | Chemistry, Physical |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1520-5207 |
| WOS关键词 | LIGHT-HARVESTING-COMPLEX ; PHOTOSYSTEM-II ; XANTHOPHYLLS ; CAROTENOIDS ; PLANTS ; PHOTOINHIBITION ; IDENTIFICATION ; INTERFACE ; PROTEINS ; SWITCH |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000789043800007 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | Natural Science Foundation of China (NSFC) [22003075, 22173115] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28568]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.[Yamano, Nami 2.Renmin Univ China, Dept Chem, Key Lab Adv Light Convers Mat & Biophoton, Beijing 100872, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Wang, Peng |
| 推荐引用方式 GB/T 7714 | Yamano, Nami,Wang, Peng,Dong, Feng-Qin,et al. Lipid-Enhanced Photoprotection of LHCII in Membrane Nanodisc by Reducing Chlorophyll Triplet Production[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2022,126(14):2669-2676. |
| APA | Yamano, Nami,Wang, Peng,Dong, Feng-Qin,&Zhang, Jian-Ping.(2022).Lipid-Enhanced Photoprotection of LHCII in Membrane Nanodisc by Reducing Chlorophyll Triplet Production.JOURNAL OF PHYSICAL CHEMISTRY B,126(14),2669-2676. |
| MLA | Yamano, Nami,et al."Lipid-Enhanced Photoprotection of LHCII in Membrane Nanodisc by Reducing Chlorophyll Triplet Production".JOURNAL OF PHYSICAL CHEMISTRY B 126.14(2022):2669-2676. |
入库方式： OAI收割
来源：植物研究所
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