Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat
文献类型：期刊论文
| 作者 | Jiang, Hao; Fang, Yu; Yan, Dong; Liu, Si-tong; Wei, Jun; Guo, Fei-long; Wu, Xing-ting; Cao, Hong; Yin, Chang-bin; Lu, Fei3,4 |
| 刊名 | THEORETICAL AND APPLIED GENETICS
 |
| 出版日期 | 2022 |
| 卷号 | 135期号:9页码:3265-3276 |
| ISSN号 | 0040-5752 |
| DOI | 10.1007/s00122-022-04184-y |
| 文献子类 | Article |
| 英文摘要 | Key message Twelve QTL associated with pre-harvest sprouting tolerance were identified using association analysis in wheat. Two markers were validated and a candidate gene TaNAC074 for Qgpf.cas-3B.2 was verified using Agrobacterium-mediated transformation. Pre-harvest sprouting (PHS) is a considerable global threat to wheat yield and quality. Due to this threat, breeders must identify quantitative trait loci (QTL) and genes conferring PHS-tolerance (PHST) to reduce the negative effects of PHS caused by low seed dormancy. In this study, we evaluated a panel of 302 diverse wheat genotypes for PHST in four environments and genotyped the panel with a high-density wheat 660 K SNP array. By using a genome-wide association study (GWAS), we identified 12 stable loci significantly associated with PHST (P < 0.0001), explaining 3.34 - 9.88% of the phenotypic variances. Seven of these loci co-located with QTL and genes reported previously. Five loci (Qgpf.cas-3B.2, Qgpf.cas-3B.3, Qgpf.cas-3B.4, Qgpf.cas-7B.2, and Qgpf.cas-7B.3), located in genomic regions with no known PHST QTL or genes, are likely to be new QTL conferring PHST. Additionally, two molecular markers were developed for Qgpf.cas-3A and Qgpf.cas-7B.3, and validated using a different set of 233 wheat accessions. Finally, the PHST-related function of candidate gene TaNAC074 for Qgpf.cas-3B.2 was confirmed by CAPS (cleaved amplified polymorphic sequences) marker association analysis in 233 wheat accessions and by expression and phenotypic analysis of transgenic wheat. Overexpression of TaNAC074 significantly reduced seed dormancy in wheat. This study contributes to broaden the genetic basis and molecular marker-assisted breeding of PHST. |
| 学科主题 | Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture |
| 电子版国际标准刊号 | 1432-2242 |
| 出版地 | NEW YORK |
| WOS关键词 | QUANTITATIVE TRAIT LOCI ; SEED-GERMINATION ; RESISTANCE ; DORMANCY ; GENES ; SELECTION ; CLONING ; FAMILY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000830275100001 |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010104] ; National Natural Science Foundation of China [32001431, U20A2033, 31870305] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28591]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Beijing 100081, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 5.Chinese Acad Sci, CAS JIC Ctr Excellence Plant & Microbial Sci CEPA, Inst Genet & Dev Biol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Hao,Fang, Yu,Yan, Dong,et al. Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat[J]. THEORETICAL AND APPLIED GENETICS,2022,135(9):3265-3276. |
| APA | Jiang, Hao.,Fang, Yu.,Yan, Dong.,Liu, Si-tong.,Wei, Jun.,...&Liu, Yong-xiu.(2022).Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat.THEORETICAL AND APPLIED GENETICS,135(9),3265-3276. |
| MLA | Jiang, Hao,et al."Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat".THEORETICAL AND APPLIED GENETICS 135.9(2022):3265-3276. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。