OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm
文献类型：期刊论文
| 作者 | Feng, Tingting3; Wang, Lili3; Li, Laiyun3; Liu, Yuan; Chong, Kang; Theissen, Guenter2; Meng, Zheng |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2022 |
| 卷号 | 234期号:1页码:77-92 |
| ISSN号 | 0028-646X |
| 关键词 | endosperm MADS-box gene NF-YB1 Oryza sativa OsMADS14 protein-protein interaction starch synthesis |
| DOI | 10.1111/nph.17990 |
| 文献子类 | Article |
| 英文摘要 | Starch synthesis makes a dramatic contribution to the yield and nutritional value of cereal crops. Although several starch synthesis enzymes and related regulators have been reported, the underlying regulatory mechanisms of starch synthesis remain largely unknown. OsMADS14 is a FRUITFULL (FUL)-like MADS-box gene in rice (Oryza sativa). Here we show that two null mutations of OsMADS14 result in a shrunken and chalky grain phenotype. It is caused by obviously defective compound starch granules and a significantly reduced content of both total starch and amylose in the endosperm. Transcriptomic profiling analyses revealed that the loss-of-function of OsMADS14 leads to significantly downregulated expression of many core starch synthesis genes, including OsAGPL2 and Waxy. Both in vitro and in vivo assays demonstrate that the OsMADS14 protein directly binds to stretches of DNA with a CArG-box consensus in the putative regulatory regions of OsAGPL2 and Waxy. Protein-protein interaction experiments also suggest that OsMADS14 interacts with nuclear factor NF-YB1 to promote the transcription of OsAGPL2 and Waxy. Our study thus demonstrates that OsMADS14 plays an essential role in the synthesis of storage starch and provides novel insights into the underlying molecular mechanism that may be used to improve rice cultivars by molecular breeding. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1469-8137 |
| 出版地 | HOBOKEN |
| WOS关键词 | ADP-GLUCOSE PYROPHOSPHORYLASE ; MADS-BOX GENES ; TRANSCRIPTION FACTORS ; MERISTEM IDENTITY ; AMYLOSE CONTENT ; PROTEIN ; BIOSYNTHESIS ; ARABIDOPSIS ; EXPRESSION ; FRUITFULL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000754301500001 |
| 资助机构 | National Natural Science Foundation of China [31771364] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28665]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Friedrich Schiller Univ Jena, Matthias Schleiden Inst, Genet, D-07743 Jena, Germany 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Tingting,Wang, Lili,Li, Laiyun,et al. OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm[J]. NEW PHYTOLOGIST,2022,234(1):77-92. |
| APA | Feng, Tingting.,Wang, Lili.,Li, Laiyun.,Liu, Yuan.,Chong, Kang.,...&Meng, Zheng.(2022).OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm.NEW PHYTOLOGIST,234(1),77-92. |
| MLA | Feng, Tingting,et al."OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm".NEW PHYTOLOGIST 234.1(2022):77-92. |
入库方式： OAI收割
来源：植物研究所
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