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Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua

文献类型：期刊论文

作者Huang, Liufang; Hu, Yonger; Huang, Ruoshi; Chen, Jiabo; Zhang, Xiande; Yue, Jingyang; Feng, Laibao3; She, Yaru; Ji, Aijia; Zheng, Ying2
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2022
关键词oxidosqualene cyclases (OSCs) Camellia sasanqua triterpene biosynthesis catalytic mechanism
ISSN号0021-8561
DOI10.1021/acs.jafc.2c03011
文献子类Article; Early Access
英文摘要Camellia sasanqua is an important economic plant that is rich in lipophilic triterpenols with pharmacological activities including antiallergic, anti-inflammatory, and anticancer activities. However, the key enzymes related to triterpene biosynthesis have seldom been studied in C. sasanqua. Oxidosqualene cyclases (OSCs) are the rate-limiting enzymes related to triterpene biosynthesis. In this study, seven putative OSC genes (CsOSC1-7) were mined from the C. sasanqua transcriptome. Six CsOSCs were characterized for the biosynthesis of diverse triterpene skeletons, including alpha-amyrin, beta-amyrin, delta-amyrin, dammarenediol-II, psi taraxasterol, taraxasterol, and cycloartenol by the heterologous expression system. CsOSC3 was a multiple functional alpha-amyrin synthase. Three key residues, Trp260, Tyr262, and Phe415, are critical to the catalytic performance of CsOSC3 judging from the results of molecular docking and site-directed mutagenesis. These findings provide important insights into the biosynthesis pathway of triterpenes in C. sasanqua.
学科主题Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
出版地WASHINGTON
电子版国际标准刊号1520-5118
WOS关键词FLOWER BUDS ; SYNTHASE ; ALCOHOLS ; EXPRESSION ; PLANT ; OILS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000821821000001
出版者AMER CHEMICAL SOC
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28807]  
专题中科院植物分子生理学重点实验室
作者单位1.Guangzhou Univ Chinese Med, Basic Med Sci Coll, Res Ctr Basic Integrat Med, Guangzhou 510006, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
3.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Int Inst Translat Chinese Med, Joint Lab Translat Canc Res Chinese Med,Minist Ed, Guangzhou 510006, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Liufang,Hu, Yonger,Huang, Ruoshi,et al. Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022.
APA Huang, Liufang.,Hu, Yonger.,Huang, Ruoshi.,Chen, Jiabo.,Zhang, Xiande.,...&Duan, Lixin.(2022).Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA Huang, Liufang,et al."Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022).

入库方式： OAI收割

来源：植物研究所

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