Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis
文献类型：期刊论文
| 作者 | Fan, Lusheng; Zhang, Cui1 ; Gao, Bin; Zhang, Yong; Stewart, Ethan4; Jez, Jakub4; Nakajima, Keiji3; Chen, Xuemei
|
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2022 |
| 卷号 | 57期号:8页码:995-1008 |
| ISSN号 | 1534-5807 |
| DOI | 10.1016/j.devcel.2022.03.015 |
| 文献子类 | Article |
| 英文摘要 | Mobile microRNAs (miRNAs) serve as local and long-distance signals in the developmental patterning and stress responses in plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations that disrupt microtubule dynamics are specifically defective for the non-cell autonomous actions of mobile miRNAs, including miR165/6 that is produced in the endodermis and moves to the vasculature to pattern xylem cell fates in Arabidopsis roots. We show that KTN1, a subunit of a microtubule-severing enzyme, is required in source cells to inhibit the loading of miR165/6 into ARGONUATE1 (AGO1), which is cell autonomous, to enable the miRNA to exit the cell. Microtubule disruption enhances the association of miR165/6 with AGO1 in the cytoplasm. These findings suggest that although cell-autonomous miRNAs load onto AGO1 in the nucleus, the cytoplasmic AGO1 loading of mobile miRNAs is a key step regulated by microtubules to promote the range of miRNA cell-to-cell movement. |
| 学科主题 | Cell Biology ; Developmental Biology |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 1878-1551 |
| WOS关键词 | SMALL RNAS ; MOBILE ; MOVEMENT ; DIFFERENTIATION ; SPECIFICATION ; BIOSYNTHESIS ; LOCALIZATION ; BIOGENESIS ; NETWORK ; MIRNAS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000832106600005 |
| 出版者 | CELL PRESS |
| 资助机构 | National Institutes of Health [GM129373] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28889]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA 2.Nara Inst Sci & Technol, Grad Sch Sci & Technol, 8916-5 Takayama, Nara 6300192, Japan 3.Vienna Bioctr Core Facil VBCF, Vienna, Austria 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Lusheng,Zhang, Cui,Gao, Bin,et al. Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis[J]. DEVELOPMENTAL CELL,2022,57(8):995-1008. |
| APA | Fan, Lusheng.,Zhang, Cui.,Gao, Bin.,Zhang, Yong.,Stewart, Ethan.,...&Chen, Xuemei.(2022).Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis.DEVELOPMENTAL CELL,57(8),995-1008. |
| MLA | Fan, Lusheng,et al."Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis".DEVELOPMENTAL CELL 57.8(2022):995-1008. |
入库方式： OAI收割
来源：植物研究所
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