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An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

文献类型：期刊论文

作者Feng, Yixuan2; Wang, Tai2,3; Liu, Lingtong
刊名JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
出版日期2022
期号187
ISSN号1940-087X
DOI10.3791/64445
文献子类Article
英文摘要Tomato (Solanum lycopersicum L.) is one of the major cash crops worldwide. The tomato seed is an important model for studying genetics and developmental biology during plant reproduction. Visualization of finer embryonic structure within a tomato seed is often hampered by seed coat mucilage, multi-cell-layered integument, and a thick-walled endosperm, which needs to be resolved by laborious embeddingsectioning. A simpler alternative is to employ tissue clearing techniques that turn the seed almost transparent using chemical agents. Although conventional clearing procedures allow deep insight into smaller seeds with a thinner seed coat, clearing tomato seeds continues to be technically challenging, especially in the late developmental stages. Presented here is a rapid and labor-saving clearing protocol to observe tomato seed development from 3 to 23 days after flowering when embryonic morphology is nearly complete. This method combines chloral hydrate-based clearing solution widely used in Arabidopsis with other modifications, including the omission of Formalin-Aceto-Alcohol (FAA) fixation, the addition of sodium hypochlorite treatment of seeds, removal of the softened seed coat mucilage, and washing and vacuum treatment. This method can be applied for efficient clearing of tomato seeds at different developmental stages and is useful in full monitoring of the developmental process of mutant seeds with good spatial resolution. This clearing protocol may also be applied to deep imaging of other commercially important species in the Solanaceae.
学科主题Multidisciplinary Sciences
出版地CAMBRIDGE
WOS关键词ENDOSPERM DEVELOPMENT ; FRUIT-GROWTH ; EMBRYO ; AUXIN ; GIBBERELLIN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者JOURNAL OF VISUALIZED EXPERIMENTS
WOS记录号WOS:000909465300044
资助机构National Natural Science Foundation of China [31870299] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/29057]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Feng, Yixuan,Wang, Tai,Liu, Lingtong. An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2022(187).
APA Feng, Yixuan,Wang, Tai,&Liu, Lingtong.(2022).An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.).JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(187).
MLA Feng, Yixuan,et al."An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .187(2022).

入库方式： OAI收割

来源：植物研究所

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