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Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice

文献类型：期刊论文

作者Chen, Liangke1; Dong, Xiangbai; Yang, Huifang1; Chai, Yaru1; Xia, Yan1; Tian, Lihong; Qu, Le Qing1
刊名PLANT PHYSIOLOGY
出版日期2023
卷号191期号:1页码:96-109
ISSN号0032-0889
DOI10.1093/plphys/kiac496
文献子类Article
英文摘要Cytosolic DISPROPORTIONATING ENZYME2 converts maltose to glucose, providing energy and cellular materials for pollen germination and pollen tube elongation in rice. Degradation of starch accumulated in pollen provides energy and cellular materials for pollen germination and pollen tube elongation. Little is known about the function of cytosolic disproportionating enzyme2 (DPE2) in rice (Oryza sativa). Here, we obtained several DPE2 knockout mutant (dpe2) lines via genomic editing and found that the mutants grew and developed normally but with greatly reduced seed-setting rates. Reciprocal crosses between dpe2 and wild-type plants demonstrated that the mutant was male sterile. In vitro and in vivo examinations revealed that the pollen of the dpe2 mutant developed and matured normally but was defective in germination and elongation. DPE2 deficiency increased maltose content in pollen, whereas it reduced the levels of starch, glucose, fructose, and adenosine triphosphate (ATP). Exogenous supply of glucose or ATP to the germination medium partially rescued the pollen germination defects of dpe2. The expression of cytosolic phosphorylase2 (Pho2) increased significantly in dpe2 pollen. Knockout of Pho2 resulted in a semi-sterile phenotype. We failed to obtain homozygous dpe2 pho2 double mutant lines. Our results demonstrate that maltose catalyzed by DPE2 to glucose is the main energy source for pollen germination and pollen tube elongation, while Pho2 might partially compensate for deficiency of DPE2.
学科主题Plant Sciences
电子版国际标准刊号1532-2548
出版地CARY
WOS关键词MALTOSE METABOLISM ; STARCH SYNTHESIS ; GENE-EXPRESSION ; SUCROSE ; LEAVES ; DEGRADATION ; MATURATION ; REPRESSION ; ANTHER ; LEADS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者OXFORD UNIV PRESS INC
WOS记录号WOS:000879842400001
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010401] ; Natural Science Foundation of China [31771697]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/29125]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Liangke,Dong, Xiangbai,Yang, Huifang,et al. Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice[J]. PLANT PHYSIOLOGY,2023,191(1):96-109.
APA Chen, Liangke.,Dong, Xiangbai.,Yang, Huifang.,Chai, Yaru.,Xia, Yan.,...&Qu, Le Qing.(2023).Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice.PLANT PHYSIOLOGY,191(1),96-109.
MLA Chen, Liangke,et al."Cytosolic disproportionating enzyme2 is essential for pollen germination and pollen tube elongation in rice".PLANT PHYSIOLOGY 191.1(2023):96-109.

入库方式： OAI收割

来源：植物研究所

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