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Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice

文献类型：期刊论文

作者Guo, Xiaoyu4; Zhang, Dajian4; Wang, Zhongliang4; Xu, Shujuan4; Batistic, Oliver3; Steinhorst, Leonie3; Li, Hao6; Weng, Yuxiang6; Ren, Dongtao1; Kudla, Jorg3
刊名EMBO JOURNAL
出版日期2023
卷号42期号:1
关键词Calcium signal Calreticulin Cold stress OsCIPK7 OsCRT3
ISSN号0261-4189
DOI10.15252/embj.2021110518
文献子类Article
英文摘要Unusually low temperatures caused by global climate change adversely affect rice production. Sensing cold to trigger signal network is a key base for improvement of chilling tolerance trait. Here, we report that Oryza sativa Calreticulin 3 (OsCRT3) localized at the endoplasmic reticulum (ER) exhibits conformational changes under cold stress, thereby enhancing its interaction with CBL-interacting protein kinase 7 (OsCIPK7) to sense cold. Phenotypic analyses of OsCRT3 knock-out mutants and transgenic overexpression lines demonstrate that OsCRT3 is a positive regulator in chilling tolerance. OsCRT3 localizes at the ER and mediates increases in cytosolic calcium levels under cold stress. Notably, cold stress triggers secondary structural changes of OsCRT3 and enhances its binding affinity with OsCIPK7, which finally boosts its kinase activity. Moreover, Calcineurin B-like protein 7 (OsCBL7) and OsCBL8 interact with OsCIPK7 specifically on the plasma membrane. Taken together, our results thus identify a cold-sensing mechanism that simultaneously conveys cold-induced protein conformational change, enhances kinase activity, and Ca2+ signal generation to facilitate chilling tolerance in rice.
学科主题Biochemistry & Molecular Biology ; Cell Biology
出版地HOBOKEN
电子版国际标准刊号1460-2075
WOS关键词DNA INSERTIONAL MUTAGENESIS ; PROTEIN-KINASE SOS2 ; ENDOPLASMIC-RETICULUM ; STRESS RESPONSES ; CALCIUM SENSORS ; PLANT-CELLS ; CALCINEURIN ; DOMAIN ; SALT ; CA2+
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000879368400001
出版者WILEY
资助机构Basic Science Center Project of National Natural Science Foundation of China [31788103] ; National Key Research and Development Program [2020YFE0202300-06] ; Special Research Assistant Project of Chinese Academy of Sciences [2021000017] ; China Postdoctoral Science Foundation [2020M670519] ; Deutsche Forschungsgemeinschaft (DFG) [INST 211/565-1 FUGG, INST211/853-1 FUGG]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/29148]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing, Peoples R China
2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing, Peoples R China
6.Westfalische Wilhelms Univ, Inst Biol & Biotechnol Pflanzen, Munster, Germany
推荐引用方式
GB/T 7714		 Guo, Xiaoyu,Zhang, Dajian,Wang, Zhongliang,et al. Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice[J]. EMBO JOURNAL,2023,42(1).
APA Guo, Xiaoyu.,Zhang, Dajian.,Wang, Zhongliang.,Xu, Shujuan.,Batistic, Oliver.,...&Chong, Kang.(2023).Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.EMBO JOURNAL,42(1).
MLA Guo, Xiaoyu,et al."Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice".EMBO JOURNAL 42.1(2023).

入库方式： OAI收割

来源：植物研究所

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