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The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control

文献类型：期刊论文

作者Xing, Jiale5; Pan, Junting5; Yi, Heng5; Lv, Kang5; Gan, Qiuliang5; Wang, Meimei5; Ge, Haitao4; Huang, Xiahe4; Huang, Fang; Wang, Yingchun4
刊名PLANT CELL
出版日期2022
卷号34期号:9页码:3383-3399
ISSN号1040-4651
DOI10.1093/plcell/koac180
文献子类Article
英文摘要Photosynthesis and the biosynthesis of many important metabolites occur in chloroplasts. In these semi-autonomous organelles, the chloroplast genome encodes approximately 100 proteins. The remaining chloroplast proteins, close to 3,000, are encoded by nuclear genes whose products are translated in the cytosol and imported into chloroplasts. However, there is still no consensus on the composition of the protein import machinery including its motor proteins and on how newly imported chloroplast proteins are refolded. In this study, we have examined the function of orf2971, the largest chloroplast gene of Chlamydomonas reinhardtii. The depletion of Orf2971 causes the accumulation of protein precursors, partial proteolysis and aggregation of proteins, increased expression of chaperones and proteases, and autophagy. Orf2971 interacts with the TIC (translocon at the inner chloroplast envelope) complex, catalyzes ATP (adenosine triphosphate) hydrolysis, and associates with chaperones and chaperonins. We propose that Orf2971 is intimately connected to the protein import machinery and plays an important role in chloroplast protein quality control. Repression of Orf2971 induces accumulation of chloroplast precursor proteins and impaired chloroplast quality indicating that Orf2971 is required for protein import and chloroplast quality control.
学科主题Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号1532-298X
出版地CARY
WOS关键词OPEN READING FRAMES ; CHLAMYDOMONAS-REINHARDTII ; CHAPERONIN 60 ; AUTOPHAGY ; EXPRESSION ; REVEALS ; IMPORT ; GENE ; TRANSFORMATION ; ORGANIZATION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者OXFORD UNIV PRESS INC
WOS记录号WOS:000817126000001
资助机构National Key R&D Program of China [2019YFA0906300] ; National Natural Science Foundation of China [31870217]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28595]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
2.Univ Geneva, Dept Mol Biol, Geneva, Switzerland
3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
6.Univ Chinese Acad Sci, Sino Danish Coll, Beijing, Peoples R China
7.Univ Geneva, Dept Plant Biol, Geneva, Switzerland
推荐引用方式
GB/T 7714		 Xing, Jiale,Pan, Junting,Yi, Heng,et al. The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control[J]. PLANT CELL,2022,34(9):3383-3399.
APA Xing, Jiale.,Pan, Junting.,Yi, Heng.,Lv, Kang.,Gan, Qiuliang.,...&Yang, Wenqiang.(2022).The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control.PLANT CELL,34(9),3383-3399.
MLA Xing, Jiale,et al."The plastid-encoded protein Orf2971 is required for protein translocation and chloroplast quality control".PLANT CELL 34.9(2022):3383-3399.

入库方式： OAI收割

来源：植物研究所

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