AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Xiong, Hai-Bo; Pan, Hui-Min; Long, Qiao-Ying; Wang, Zi-Yuan; Qu, Wan-Tong; Mei, Tong; Zhang, Nan; Xu, Xiao-Feng; Yang, Zhong-Nan; Yu, Qing-Bo |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2022 |
| 卷号 | 50期号:12页码:6715-6734 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkac501 |
| 文献子类 | Article |
| 英文摘要 | In Escherichia coli, transcription-translation coupling is mediated by NusG. Although chloroplasts are descendants of endosymbiotic prokaryotes, the mechanism underlying this coupling in chloroplasts remains unclear. Here, we report transcription-translation coupling through AtNusG in chloroplasts. AtNusG is localized in chloroplast nucleoids and is closely associated with the chloroplast PEP complex by interacting with its essential component PAP9. It also comigrates with chloroplast ribosomes and interacts with their two components PRPS5 (uS5c) and PRPS10 (uS10c). These data suggest that the transcription and translation machineries are coupled in chloroplasts. In the atnusg mutant, the accumulation of chloroplast-encoded photosynthetic gene transcripts, such as psbA, psbB, psbC and psbD, was not obviously changed, but that of their proteins was clearly decreased. Chloroplast polysomic analysis indicated that the decrease in these proteins was due to the reduced efficiency of their translation in this mutant, leading to reduced photosynthetic efficiency and enhanced sensitivity to cold stress. These data indicate that AtNusG-mediated coupling between transcription and translation in chloroplasts ensures the rapid establishment of photosynthetic capacity for plant growth and the response to environmental changes. Therefore, our study reveals a conserved mechanism of transcription-translation coupling between chloroplasts and E. coli, which perhaps represents a regulatory mechanism of chloroplast gene expression. This study provides insights into the underlying mechanisms of chloroplast gene expression in higher plants. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1362-4962 |
| WOS关键词 | ACTIVE CHROMOSOME COMPLEX ; RIBOSOMAL-PROTEIN ; FACTOR-NUSG ; INTERACTS ; STRESS ; VISUALIZATION ; COMPONENT ; PLASTIDS ; SEQUENCE ; BINDING |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000814749800001 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Scientific Foundation of China [31570232, 31930009] ; Shanghai Key Laboratory of Plant Molecular Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28647]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Zhang, Nan 2.[Xiong, Hai-Bo 3.Pan, Hui-Min 4.Long, Qiao-Ying 5.Wang, Zi-Yuan 6.Qu, Wan-Tong 7.Mei, Tong 8.Xu, Xiao-Feng 9.Yang, Zhong-Nan 10.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiong, Hai-Bo,Pan, Hui-Min,Long, Qiao-Ying,et al. AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana[J]. NUCLEIC ACIDS RESEARCH,2022,50(12):6715-6734. |
| APA | Xiong, Hai-Bo.,Pan, Hui-Min.,Long, Qiao-Ying.,Wang, Zi-Yuan.,Qu, Wan-Tong.,...&Yu, Qing-Bo.(2022).AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana.NUCLEIC ACIDS RESEARCH,50(12),6715-6734. |
| MLA | Xiong, Hai-Bo,et al."AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana".NUCLEIC ACIDS RESEARCH 50.12(2022):6715-6734. |
入库方式： OAI收割
来源：植物研究所
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