Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II
文献类型：期刊论文
| 作者 | Han, Guangye1; Chernev, Petko; Styring, Stenbjorn; Messinger, Johannes2; Mamedov, Fikret |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2022 |
| 卷号 | 13期号:29页码:8667-8678 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/d2sc00854h |
| 文献子类 | Article |
| 英文摘要 | Photosynthesis stores solar light as chemical energy and efficiency of this process isv highly important. The electrons required for CO2 reduction are extracted from water in a reaction driven by light-induced charge separations in the Photosystem II reaction center and catalyzed by the CaMn4O5-cluster. This cyclic process involves five redox intermediates known as the S-0-S-4 states. In this study, we quantify the flash-induced turnover efficiency of each S state by electron paramagnetic resonance spectroscopy. Measurements were performed in photosystem II membrane preparations from spinach in the presence of an exogenous electron acceptor at selected temperatures between -10 degrees C and +20 degrees C and at flash frequencies of 1.25, 5 and 10 Hz. The results show that at optimal conditions the turnover efficiencies are limited by reactions occurring in the water oxidizing complex, allowing the extraction of their S state dependence and correlating low efficiencies to structural changes and chemical events during the reaction cycle. At temperatures 10 degrees C and below, the highest efficiency (i.e. lowest miss parameter) was found for the S-1 -> S-2 transition, while the S-2 -> S-3 transition was least efficient (highest miss parameter) over the whole temperature range. These electron paramagnetic resonance results were confirmed by measurements of flash-induced oxygen release patterns in thylakoid membranes and are explained on the basis of S state dependent structural changes at the CaMn4O5-cluster that were determined recently by femtosecond X-ray crystallography. Thereby, possible molecular errors connected to the e(-) transfer, H+ transfer, H2O binding and O-2 release are identified. |
| 学科主题 | Chemistry, Multidisciplinary |
| 出版地 | CAMBRIDGE |
| 电子版国际标准刊号 | 2041-6539 |
| WOS关键词 | OXYGEN-EVOLVING COMPLEX ; RAY-ABSORPTION SPECTROSCOPY ; S-STATE TRANSITIONS ; PHOTOSYNTHETIC O2 EVOLUTION ; SP PCC 6803 ; MANGANESE COMPLEX ; P680(+CENTER-DOT) REDUCTION ; TEMPERATURE-DEPENDENCE ; CENTERED OXIDATION ; STRUCTURAL-CHANGES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000823810200001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | Swedish Research Council [2020-03809] ; NordForsk [82845] ; Swedish Research Council [2020-03809] Funding Source: Swedish Research Council ; NordForsk [82845] Funding Source: researchfish |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28681]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Umea Univ, Dept Chem, S-90187 Umea, Sweden 2.Uppsala Univ, Dept Chem, Angstrom Lab, Mol Biomimet, Box 523, S-75120 Uppsala, Sweden 3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Guangye,Chernev, Petko,Styring, Stenbjorn,et al. Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II[J]. CHEMICAL SCIENCE,2022,13(29):8667-8678. |
| APA | Han, Guangye,Chernev, Petko,Styring, Stenbjorn,Messinger, Johannes,&Mamedov, Fikret.(2022).Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II.CHEMICAL SCIENCE,13(29),8667-8678. |
| MLA | Han, Guangye,et al."Molecular basis for turnover inefficiencies (misses) during water oxidation in photosystem II".CHEMICAL SCIENCE 13.29(2022):8667-8678. |
入库方式： OAI收割
来源：植物研究所
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