Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center
文献类型:期刊论文
作者 | Fang, Yuan1; Liu, Dongyang1; Jiang, Jingjing; He, Axin2; Zhu, Rui3; Tian, Lijin |
刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
出版日期 | 2022 |
卷号 | 298期号:4 |
DOI | 10.1016/j.jbc.2022.101783 |
文献子类 | Article |
英文摘要 | Photosynthetic organisms have evolved light-harvesting antennae over time. In cyanobacteria, external phycobilisomes (PBSs) are the dominant antennae, whereas in green algae and higher plants, PBSs have been replaced by proteins of the Lhc family that are integrated in the membrane. Red algae represent an evolutionary intermediate between these two systems, as they employ both PBSs and membrane LHCR proteins as light-harvesting units. Understanding how red algae cope with light is not only interesting for biotechnological applications, but is also of evolutionary interest. For example, energy-dependent quenching (qE) is an essential photoprotective mechanism widely used by species from cyanobacteria to higher plants to avoid light damage; however, the quenching mechanism in red algae remains largely unexplored. Here, we used both pulse amplitude-modulated (PAM) and time-resolved chlorophyll fluorescence to characterize qE kinetics in the red alga Porphyridium purpureum. PAM traces confirmed that qE in P. purpureum is activated by a decrease in the thylakoid lumen pH, whereas time-resolved fluorescence results further revealed the quenching site and ultrafast quenching kinetics. We found that quenching exclusively takes place in the photosystem II (PSII) complexes and preferentially occurs at PSII's core antenna rather than at its reaction center, with an overall quenching rate of 17.6 +/- 3.0 ns(-1). In conclusion, we propose that qE in red algae is not a reaction center type of quenching, and that there might be a membrane-bound protein that resembles PsbS of higher plants or LHCSR of green algae that senses low luminal pH and triggers qE in red algae. |
学科主题 | Biochemistry & Molecular Biology |
电子版国际标准刊号 | 1083-351X |
出版地 | AMSTERDAM |
WOS关键词 | LIGHT-HARVESTING COMPLEXES ; CHLOROPHYLL FLUORESCENCE ; CHLAMYDOMONAS-REINHARDTII ; STATE TRANSITIONS ; EXCESS LIGHT ; PROTEIN ; DISSIPATION ; CYANOBACTERIAL ; MECHANISMS ; MEMBRANES |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000823106100009 |
资助机构 | National Key Research and Development Program of China [2019YFA0904604] ; National Natural Science Foundation of China [11804172, 31970381, 11605001, 91850104] |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28803] |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Peking Univ, Sch Phys, State Key Lab Artificial Microstruct & Mesoscop P, Beijing, Peoples R China 4.Peking Univ, Sch Phys, Electron Microscopy Lab, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Fang, Yuan,Liu, Dongyang,Jiang, Jingjing,et al. Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2022,298(4). |
APA | Fang, Yuan,Liu, Dongyang,Jiang, Jingjing,He, Axin,Zhu, Rui,&Tian, Lijin.(2022).Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center.JOURNAL OF BIOLOGICAL CHEMISTRY,298(4). |
MLA | Fang, Yuan,et al."Photoprotective energy quenching in the red alga Porphyridium purpureum occurs at the core antenna of the photosystem II but not at its reaction center".JOURNAL OF BIOLOGICAL CHEMISTRY 298.4(2022). |
入库方式: OAI收割
来源:植物研究所
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