Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser
文献类型：期刊论文
| 作者 | Liu, Xiaohong; Liu, Pengcheng; Li, Hongjie; Xu, Zhen; Jia, Lu; Xia, Yan; Yu, Minling; Tang, Wenqin; Zhu, Xiaolei; Chen, Chao |
| 刊名 | NATURE CHEMISTRY
 |
| 出版日期 | 2022 |
| 卷号 | 14期号:9页码:1054+ |
| ISSN号 | 1755-4330 |
| DOI | 10.1038/s41557-022-00992-3 |
| 文献子类 | Article |
| 英文摘要 | One of the primary objectives in chemistry research is to observe atomic motions during reactions in real time. Although X-ray free-electron lasers (XFELs) have facilitated the capture of reaction intermediates using time-resolved serial femtosecond crystallography (TR-SFX), only a few natural photoactive proteins have been investigated using this method, mostly due to the lack of suitable phototriggers. Here we report the genetic encoding of a xanthone amino acid (FXO), as an efficient phototrigger, into a rationally designed human liver fatty-acid binding protein mutant (termed XOM), which undergoes photo-induced C-H bond transformation with high selectivity and quantum efficiency. We solved the structures of XOM before and 10-300 ns after flash illumination, at 1.55-1.70 angstrom resolutions, and captured the elusive excited-state intermediates responsible for precise C-H bond activation. We expect that most redox enzymes can now be investigated by TR-SFX, using our method, to reveal reaction intermediates key for their efficiency and selectivity. |
| 学科主题 | Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1755-4349 |
| 出版地 | BERLIN |
| WOS关键词 | EXCIPLEX FORMATION ; STRUCTURAL-CHANGES ; CRYSTALLOGRAPHY ; MYOGLOBIN ; PATHWAYS ; EXCIMERS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | NATURE PORTFOLIO |
| WOS记录号 | WOS:000826794700003 |
| 资助机构 | National Science Foundation of China [22193023, 22121003, 91953202, 21837005] ; National Key Research and Development Program of China [2017YFA0503704, 2017YFA0503700, 2019YFA0904101] ; CAS Project for Young Scientists in Basic Research [YSBR-015] ; Sanming Project of Medicine in Shenzhen [SZSM201811092] ; Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101070] ; JSPS KAKENHI [JP18H02394] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28890]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Kyoto Univ, Grad Sch Med, Dept Cell Biol, Kyoto, Japan 2.RIKEN SPring 8 Ctr, Kobe, Hyogo, Japan 3.Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Sendai, Miyagi, Japan 4.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan 5.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan 6.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing, Peoples R China 7.Univ Hyogo, Grad Sch Life Sci, Dept Picobiol, Kobe, Hyogo, Japan 8.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 9.Japan Synchrotron Radiat Res Inst, Kobe, Hyogo, Japan |
| 推荐引用方式 GB/T 7714 | Liu, Xiaohong,Liu, Pengcheng,Li, Hongjie,et al. Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser[J]. NATURE CHEMISTRY,2022,14(9):1054+. |
| APA | Liu, Xiaohong.,Liu, Pengcheng.,Li, Hongjie.,Xu, Zhen.,Jia, Lu.,...&Wang, Jiangyun.(2022).Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser.NATURE CHEMISTRY,14(9),1054+. |
| MLA | Liu, Xiaohong,et al."Excited-state intermediates in a designer protein encoding a phototrigger caught by an X-ray free-electron laser".NATURE CHEMISTRY 14.9(2022):1054+. |
入库方式： OAI收割
来源：植物研究所
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