Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1
文献类型：期刊论文
| 作者 | Zhang, Chi; Zhao, Shun2,4; Li, Yu-Shuai; He, Chao1; Wang, Xiao1; Liu, Lin1 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2022 |
| 卷号 | 13 |
| 关键词 | ascorbic acid nucleotide sugar guanylyltransferase crystallography oligomerization |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2022.899738 |
| 文献子类 | Article |
| 英文摘要 | Plant GDP-D-mannose pyrophosphorylase (GMPase) catalyzes a committed step in ascorbic acid biosynthesis pathway. Arabidopsis thaliana VTC1 is the first genetically characterized plant GMPase and has unique properties when compared with bacterial and animal homologs. Here we present the crystal structures of VTC1 in the unliganded and product-bound states at resolutions of 2.8 and 3.0 angstrom, respectively. VTC1 dimerizes in a same way like other known GMPases, but dodecamerizes in a previously unobserved arrangement. The interactions to GDP-D-mannose and inorganic pyrophosphate are revealed by the product-bound VTC1 structure. An in vitro GMPase activity assay confirms the regulatory role of the C-terminal left-handed beta-helix domain, and structural analyses suggest the models of VTC1 hetero-complex with its interacting proteins. The structural information advances our insights into the different mechanisms involved in VTC1 regulation. |
| 学科主题 | Plant Sciences |
| 出版地 | LAUSANNE |
| WOS关键词 | L-ASCORBIC-ACID ; STRUCTURE REFINEMENT ; BIOSYNTHESIS ; METABOLISM ; DEFICIENT ; MUTANT ; MODEL ; CYTOKINESIS ; AMMONIUM ; MUTATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000807112400001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28997]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China 3.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY USA 4.Anhui Univ, Sch Life Sci, Hefei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,et al. Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1[J]. FRONTIERS IN PLANT SCIENCE,2022,13. |
| APA | Zhang, Chi,Zhao, Shun,Li, Yu-Shuai,He, Chao,Wang, Xiao,&Liu, Lin.(2022).Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1.FRONTIERS IN PLANT SCIENCE,13. |
| MLA | Zhang, Chi,et al."Crystal Structures of Arabidopsis thaliana GDP-D-Mannose Pyrophosphorylase VITAMIN C DEFECTIVE 1".FRONTIERS IN PLANT SCIENCE 13(2022). |
入库方式： OAI收割
来源：植物研究所
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