A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes
文献类型：期刊论文
| 作者 | Gao, Zhi-Fang; Yang, Xiu; Mei, Yingchang; Zhang, Jiao; Chao, Qing; Wang, Bai-Chen |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2023 |
| 卷号 | 113期号:2页码:291-307 |
| 关键词 | maize C4 phosphoproteome kinase-substrate network light and temperature |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.16047 |
| 文献子类 | Article |
| 英文摘要 | As sessile organisms, plants need to respond to rapid changes in numerous environmental factors, mainly diurnal changes of light, temperature, and humidity. Maize is the world's most grown crop, and as a C4 plant it exhibits high photosynthesis capacity, reaching the highest rate of net photosynthesis at midday; that is, there is no midday depression. Revealing the physiological responses to diurnal changes and underlying mechanisms will be of great significance for guiding maize improvement efforts. In this study, we collected maize leaf samples and analyzed the proteome and phosphoproteome at nine time points during a single day/night cycle, quantifying 7424 proteins and 5361 phosphosites. The new phosphosites identified in our study increased the total maize phosphoproteome coverage by 8.5%. Kinase-substrate network analysis indicated that 997 potential substrates were phosphorylated by 20 activated kinases. Through analysis of proteins with significant changes in abundance and phosphorylation, we found that the response to a heat stimulus involves a change in the abundance of numerous proteins. By contrast, the high light at noon and rapidly changing light conditions induced changes in the phosphorylation level of proteins involved in processes such as chloroplast movement, photosynthesis, and C4 pathways. Phosphorylation is involved in regulating the activity of large number of enzymes; for example, phosphorylation of S55 significantly enhanced the activity of maize phosphoenolpyruvate carboxykinase1 (ZmPEPCK1). Overall, the database of dynamic protein abundance and phosphorylation we have generated provides a resource for the improvement of C4 crop plants. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | BUNDLE-SHEATH CHLOROPLASTS ; PHOTOSYSTEM-II ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; LEAF PHOTOSYNTHESIS ; REGULATORY PHOSPHORYLATION ; SUCROSE TRANSPORTER ; SEEDLING LEAVES ; HEAT-STRESS ; ARABIDOPSIS ; LIGHT |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000898713500001 |
| 出版者 | WILEY |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; [XDA24010203] ; [2021YFA1300403] ; [31671710] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29097]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.[Chao, Qing 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China 4.Inst Bot, Chinese Acad Sci, Key Lab Photobiol, CAS, Beijing 100093, Peoples R China 5.Chao, Qing 6.Zhang, Jiao 7.Mei, Yingchang 8.Yang, Xiu 9.[Gao, Zhi-Fang |
| 推荐引用方式 GB/T 7714 | Gao, Zhi-Fang,Yang, Xiu,Mei, Yingchang,et al. A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes[J]. PLANT JOURNAL,2023,113(2):291-307. |
| APA | Gao, Zhi-Fang,Yang, Xiu,Mei, Yingchang,Zhang, Jiao,Chao, Qing,&Wang, Bai-Chen.(2023).A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes.PLANT JOURNAL,113(2),291-307. |
| MLA | Gao, Zhi-Fang,et al."A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes".PLANT JOURNAL 113.2(2023):291-307. |
入库方式： OAI收割
来源：植物研究所
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