Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling
文献类型：期刊论文
| 作者 | Li, Yuhong; Liu, Hanhong; Ma, Tingting; Li, Jialong; Yuan, Jiarui; Xu, Yong-Chao; Sun, Ran; Zhang, Xinyu4; Jing, Yanjun; Guo, Ya-Long |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2023 |
| 卷号 | 35期号:2页码:827-851 |
| ISSN号 | 1040-4651 |
| DOI | 10.1093/plcell/koac330 |
| 文献子类 | Article |
| 英文摘要 | Chloroplasts produce singlet oxygen (O-1(2)), which causes changes in nuclear gene expression through plastid-to-nucleus retrograde signaling to increase plant fitness. However, the identity of this O-1(2)-triggered pathway remains unclear. Here, we identify mutations in GENOMES UNCOUPLED4 (GUN4) and GUN5 as suppressors of phytochrome-interacting factor1 (pif1) pif3 in regulating the photo-oxidative response in Arabidopsis thaliana. GUN4 and GUN5 specifically interact with EXECUTER1 (EX1) and EX2 in plastids, and this interaction is alleviated by treatment with Rose Bengal (RB) or white light. Impaired expression of GUN4, GUN5, EX1, or EX2 leads to insensitivity to excess light and overexpression of EX1 triggers photo-oxidative responses. Strikingly, upon light irradiation or RB treatment, EX1 transiently accumulates in the nucleus and the nuclear fraction of EX1 shows a similar molecular weight as the plastid-located protein. Point mutagenesis analysis indicated that nuclear localization of EX1 is required for its function. EX1 acts as a transcriptional co-activator and interacts with the transcription factors WRKY18 and WRKY40 to promote the expression of O-1(2)-responsive genes. This study suggests that EX1 may act in plastid-to-nucleus signaling and establishes a O-1(2)-triggered retrograde signaling pathway that allows plants adapt to changing light environments during chloroplast development. EX1 acts as a plastid-to-nucleus mobile protein that relays O-1(2) signals, thus bridging the communication gap between plastids and the nucleus. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 电子版国际标准刊号 | 1532-298X |
| 出版地 | CARY |
| WOS关键词 | CHLOROPHYLL BIOSYNTHESIS ; GENE-EXPRESSION ; STRESS RESPONSES ; PROTEIN INTERACTIONS ; MG-CHELATASE ; DNA-BINDING ; CHLOROPLAST ; LIGHT ; REGULATOR ; REVEALS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS INC |
| WOS记录号 | WOS:000927607300001 |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29120]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.ENS Lyon, Lab Reprod & Dev Plantes, Lyon, France 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuhong,Liu, Hanhong,Ma, Tingting,et al. Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling[J]. PLANT CELL,2023,35(2):827-851. |
| APA | Li, Yuhong.,Liu, Hanhong.,Ma, Tingting.,Li, Jialong.,Yuan, Jiarui.,...&Lin, Rongcheng.(2023).Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling.PLANT CELL,35(2),827-851. |
| MLA | Li, Yuhong,et al."Arabidopsis EXECUTER1 interacts with WRKY transcription factors to mediate plastid-to-nucleus singlet oxygen signaling".PLANT CELL 35.2(2023):827-851. |
入库方式： OAI收割
来源：植物研究所
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