Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata
文献类型：期刊论文
| 作者 | Yan, Huili; Xu, Wenxiu; Zhang, Tian3; Feng, Lu; Liu, Ruoxi3; Wang, Luyao3; Wu, Lulu3; Zhang, Han3; Zhang, Xiaohan4; Li, Ting3 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2022 |
| 卷号 | 233期号:6页码:2488-2502 |
| 关键词 | AsIII efflux long-distance translocation Pteris vittata PvAsE1 SLC13-like protein |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.17962 |
| 文献子类 | Article |
| 英文摘要 | Pteris vittata is an arsenic (As) hyperaccumulator that can accumulate several thousand mg As kg(-1) DW in aboveground biomass. A key factor for its hyperaccumulation ability is its highly efficient As long-distance translocation system. However, the underlying molecular mechanisms remain unknown. We isolated PvAsE1 through the full-length cDNA over-expression library of P. vittata and characterized it through a yeast system, RNAi gametophytes and sporophytes, subcellular-location and in situ hybridization. Phylogenomic analysis was conducted to estimate the appearance time of PvAsE1. PvAsE1 was a plasma membrane-oriented arsenite (AsIII) effluxer. The silencing of PvAsE1 reduced AsIII long-distance translocation in P. vittata sporophytes. PvAsE1 was structurally similar to solute carrier (SLC)13 proteins. Its transcripts could be observed in parenchyma cells surrounding the xylem of roots. The appearance time was estimated at c. 52.7 Ma. PvAsE1 was a previously uncharacterized SLC13-like AsIII effluxer, which may contribute to AsIII long-distance translocation via xylem loading. PvAsE1 appeared late in fern evolution and might be an adaptive subject to the selection pressure at the Cretaceaou-Paleogene boundary. The identification of PvAsE1 provides clues for revealing the special As hyperaccumulation characteristics of P. vittata. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1469-8137 |
| WOS关键词 | CRETACEOUS-TERTIARY EXTINCTION ; DICARBOXYLATE CARRIER PROTEIN ; FUNCTIONAL-CHARACTERIZATION ; ACCUMULATION ; TOLERANCE ; EFFICIENT ; MECHANISMS ; SILICON ; RICE ; PHYTOREMEDIATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000748358900001 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China [31770298, 41977131] ; National Key Research and Development Program of China [2017YFD0800900] ; Innovation Training Programs for Undergraduates, CAS [20214000859] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28560]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Shanxi Agr Univ, Sorghum Res Inst, Jinzhong 030600, Peoples R China 2.Zhejiang Stn Management Arable Land Qual & Fertil, Hangzhou 310020, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Huili,Xu, Wenxiu,Zhang, Tian,et al. Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata[J]. NEW PHYTOLOGIST,2022,233(6):2488-2502. |
| APA | Yan, Huili.,Xu, Wenxiu.,Zhang, Tian.,Feng, Lu.,Liu, Ruoxi.,...&He, Zhenyan.(2022).Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata.NEW PHYTOLOGIST,233(6),2488-2502. |
| MLA | Yan, Huili,et al."Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata".NEW PHYTOLOGIST 233.6(2022):2488-2502. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。