Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge
文献类型：期刊论文
| 作者 | Zhang, Lianhua2; Wang, Jian; Piao, Xiangshu |
| 刊名 | ANTIOXIDANTS
 |
| 出版日期 | 2022 |
| 卷号 | 11期号:11 |
| 关键词 | 25-hydroxycholecalciferol blood antioxidant status intestinal health microbiota broilers |
| DOI | 10.3390/antiox11112094 |
| 文献子类 | Article |
| 英文摘要 | Our experiment was to detect the effects of 25-hydroxycholecalciferol (25OHD(3)) on antioxidant capacity, immune status and gut health of broilers under lipopolysaccharide (LPS) challenge. In total, 108 male Arbor Acre broilers (48.5 +/- 0.4 g) were allotted to three treatment groups containing six replicates for each group with six birds per replicate: (1) corn-soybean basal diet + injection of sterile saline (CON group); (2) corn-soybean basal diet + an injection of LPS (LPS group); (3) corn-soybean basal diet with 50 mu g/kg 25OHD(3) + injection of LPS (LPS + 25-D group). At the end of the experiment, birds were intraperitoneally injected with LPS in the LPS and LPS + 25-D groups based on the dosage of 5.0 mg/kg BW, or the equivalent volume of 0.9% sterile saline in the CON group. At 4 h postinjection, blood samples, jejunal and ileal tissues and cecal digesta were collected to analyze blood antioxidant capacity, intestinal barrier function and microbiota. The results showed that broilers challenged with LPS had significantly higher BW loss than the CON group, and 25OHD(3) alleviated BW loss induced by the LPS challenge. 25OHD(3) alleviated the LPS-induced decline (p < 0.05) in serum activities of superoxide dismutase (SOD) and immunoglobulin G (IgG), as well as prevented the LPS-induced increase (p < 0.05) in serum content of tumor necrosis factor-alpha (TNF-alpha). 25OHD(3) significantly increased villus height in the jejunum and the relative mRNA abundance of Occludin in the jejunum and ileum, as well as prevented the LPS-induced increase in the jejunal content of interferon-gamma (IFN-gamma) compared with the LPS group. Compared with the LPS group, 25OHD3 significantly increased Lactobacillus abundance and decreased Lachnoclostridium abundance in the cecal digesta, as well as had the potential to enhance metabolite contents including propionate, isobutyrate, butyrate and total SCFA. The correlation analysis revealed that BW loss and serum contents of TNF-alpha, IL-1 beta and D-lactate were positively correlated with Lachnoclostridium and negatively correlated with Lactobacillus (p < 0.05). Overall, 25OHD(3) partially improves the antioxidant status, immunity, intestinal barrier and microbial composition of broilers under the LPS challenge. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 2076-3921 |
| WOS关键词 | VITAMIN-D ; INFLAMMATORY RESPONSES ; 1,25-DIHYDROXYVITAMIN D-3 ; NUTRIENT DIGESTIBILITY ; PHYSIOLOGICAL STRESS ; OXIDATIVE STRESS ; SUPPLEMENTATION ; CHICKENS ; RECEPTOR ; PIGLETS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000880746100001 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China [31772612] ; Beijing Municipal Natural Science Foundation [6202019] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28709]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 2.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lianhua,Wang, Jian,Piao, Xiangshu. Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge[J]. ANTIOXIDANTS,2022,11(11). |
| APA | Zhang, Lianhua,Wang, Jian,&Piao, Xiangshu.(2022).Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge.ANTIOXIDANTS,11(11). |
| MLA | Zhang, Lianhua,et al."Potential Effects of 25-Hydroxycholecalciferol on the Growth Performance, Blood Antioxidant Capacity, Intestinal Barrier Function and Microbiota in Broilers under Lipopolysaccharide Challenge".ANTIOXIDANTS 11.11(2022). |
入库方式： OAI收割
来源：植物研究所
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