iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism of Golden-Yellow Leaf Mutant in Hybrid Paper Mulberry
文献类型：期刊论文
| 作者 | Wang, Fenfen2; Chen, Naizhi; Shen, Shihua |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2022 |
| 卷号 | 23期号:1 |
| 关键词 | chlorophyll chloroplast photosynthesis ribosome proteome |
| DOI | 10.3390/ijms23010127 |
| 文献子类 | Article |
| 英文摘要 | Plant growth and development relies on the conversion of light energy into chemical energy, which takes place in the leaves. Chlorophyll mutant variations are important for studying certain physiological processes, including chlorophyll metabolism, chloroplast biogenesis, and photosynthesis. To uncover the mechanisms of the golden-yellow phenotype of the hybrid paper mulberry plant, this study used physiological, cytological, and iTRAQ-based proteomic analyses to compare the green and golden-yellow leaves of hybrid paper mulberry. Physiological results showed that the mutants of hybrid paper mulberry showed golden-yellow leaves, reduced chlorophyll, and carotenoid content, and increased flavonoid content compared with wild-type plants. Cytological observations revealed defective chloroplasts in the mesophyll cells of the mutants. Results demonstrated that 4766 proteins were identified from the hybrid paper mulberry leaves, of which 168 proteins displayed differential accumulations between the green and mutant leaves. The differentially accumulated proteins were primarily involved in chlorophyll synthesis, carotenoid metabolism, and photosynthesis. In addition, differentially accumulated proteins are associated with ribosome pathways and could enable plants to adapt to environmental conditions by regulating the proteome to reduce the impact of chlorophyll reduction on growth and survival. Altogether, this study provides a better understanding of the formation mechanism of the golden-yellow leaf phenotype by combining proteomic approaches. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1422-0067 |
| 出版地 | BASEL |
| WOS关键词 | CHLOROPHYLL BIOSYNTHESIS ; CHLOROPLAST BIOGENESIS ; MG-CHELATASE ; SUBUNIT ; COLOR ; ACCUMULATION ; REDUCTASE ; PROTEINS ; COMPLEX ; GENE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000751247900001 |
| 资助机构 | National Natural Science Foundation of China [31870247] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28739]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fenfen,Chen, Naizhi,Shen, Shihua. iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism of Golden-Yellow Leaf Mutant in Hybrid Paper Mulberry[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(1). |
| APA | Wang, Fenfen,Chen, Naizhi,&Shen, Shihua.(2022).iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism of Golden-Yellow Leaf Mutant in Hybrid Paper Mulberry.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(1). |
| MLA | Wang, Fenfen,et al."iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism of Golden-Yellow Leaf Mutant in Hybrid Paper Mulberry".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.1(2022). |
入库方式： OAI收割
来源：植物研究所
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