Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes
文献类型：期刊论文
| 作者 | Liang, Yan2,4; Kong, Fan2,3; Ma, Xiaofeng3; Shu, Qingyan2 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2022 |
| 卷号 | 27期号:4 |
| 关键词 | fatty acid synthase Acer truncatum Bunge inhibitor obesity |
| DOI | 10.3390/molecules27041324 |
| 文献子类 | Article |
| 英文摘要 | Acer truncatum Bunge is now widely cultivated throughout the world. Fatty acid synthase (FAS) is a potential target in the treatment of both obesity and cancer. Only a few FAS inhibitors have been reported. In this study, the inhibitory effect of A. truncatum seed coat (ESA) on FAS and the inhibition mechanisms were investigated using a FAS activity assay and an enzyme kinetics study. The main chemicals of ESA were analyzed with UPLC-MS/MS. The effects of ESA on 3T3-L1 adipocyte differentiation and lipid accumulation were investigated using Oil red O staining. We first identified seven main compounds (quinic acid, malic acid, gentisic acid, procyanidin dimer, procyanidin trimer, catechin, and quercetin) from 50% ethanol extracts of seed coats of A. truncatum (ESAs), which were then found to inhibit 3T3-L1 adipocyte differentiation at the concentration of 50 mu g/mL. ESA obviously reduced the visible triglyceride droplets accumulation, and dramatically decreased the number of the adipocytes at a comparatively high concentration. It is suggested that the effects are due to the inhibition of FAS by ESA; FAS activity is inhibited by ESA at a half inhibition concentration (IC50) of 0.57 mu g/mL, which is lower than that of classically known FAS inhibitors. Meanwhile, ESA displayed different inhibition kinetics and reacting sites for FAS. These results provide new clues for the development of novel products for obesity treatment and a scientific basis for the full use of byproducts for future industrial production of vegetable oil. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1420-3049 |
| 出版地 | BASEL |
| WOS关键词 | CANCER ; LEAVES ; OBESITY ; WEIGHT ; OIL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000764362100001 |
| 资助机构 | Biological Resources Program, Chinese Academy of Sciences [KFJ-BRP-007] ; Fundamental Research Funds for Central Universities [Y95401AXX2] ; Youth Innovation Promotion Association, CAS [2012315] ; Zhongke Ruilaiyin (Beijing) Biotechnology Co., Ltd [2021C037] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28836]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Capital Univ Phys Educ & Sports, Sch Kinesiol & Hlth, 11 Beisanhuanxi Rd, Beijing 100191, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yan,Kong, Fan,Ma, Xiaofeng,et al. Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes[J]. MOLECULES,2022,27(4). |
| APA | Liang, Yan,Kong, Fan,Ma, Xiaofeng,&Shu, Qingyan.(2022).Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes.MOLECULES,27(4). |
| MLA | Liang, Yan,et al."Inhibitory Effect of Acer truncatum Bunge Seed Coat Extract on Fatty Acid Synthase, Differentiation and Lipid Accumulation in 3T3-L1 Adipocytes".MOLECULES 27.4(2022). |
入库方式： OAI收割
来源：植物研究所
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