Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium
文献类型：期刊论文
| 作者 | Hao, Yuanpeng2; Guo, Xiaoqi2; Zhang, Wenying2; Xia, Fei; Yang, Evan1; Li, Hui; Bai, Hongtong; Shi, Lei
|
| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
 |
| 出版日期 | 2022 |
| 卷号 | 189 |
| 关键词 | Rosemary essential oil Salmonella enterica serovar Typhimurium Antimicrobial mechanism Growth parameters Proteomics |
| ISSN号 | 0926-6690 |
| DOI | 10.1016/j.indcrop.2022.115757 |
| 文献子类 | Article |
| 英文摘要 | Rosemary essential oil (REO) shows various biological functions and is widely used in the cosmetic and pharmaceutical industries. Salmonella enterica serovar Typhimurium infection treated by antibiotics leads to serious drug resistance and public health hazard. REO as an antibiotic alternative has become the focus of considerable research efforts. However, knowledge regarding the S. Typhimurium growth characteristics and proteomic responses to REO exposure remains unclear. Here, we evaluated the commonalities and differences in chemical compositions of REOs extracted from Salvia rosmarinus cultivars, 'Algarve' (AL), 'Dutch Mill' (DM), and 'Majorca Pink' (MP) using gas chromatography-mass spectrometry. The three REOs displayed distinct chemical composition profiles, and the DM-REO, predominantly composed of 1,8-cineole and alpha-pinene, had the highest antibacterial activity. Sublethal levels of DM-REO caused irreversible cell membrane damage, longer lag phases, decreased growth rates, and lower maximum optical densities of S. Typhimurium. The underlying molecular regulatory mechanisms of S. Typhimurium under sublethal DM-REO treatment were investigated using label-free quantitative proteomics. Four hundred thirty-six differentially expressed proteins mainly involved in metabolism, ABC transporters, two-component system, quorum sensing, bacterial chemotaxis, and flagellar assembly were identified. The phenotypic observation test showed that DM-REO significantly inhibited S. Typhimurium motility and quorum sensing. These results increase our understanding of the antibacterial mechanism of REO inhibition in S. Typhimurium. |
| 学科主题 | Agricultural Engineering ; Agronomy |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1872-633X |
| WOS关键词 | ROSMARINUS-OFFICINALIS ; ANTIOXIDANT PROPERTIES ; L. |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000906234400003 |
| 出版者 | ELSEVIER |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080603] ; R&D Program of Sinno Cosmetics Co., Ltd [2019C079] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28846]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Sinno Cosmet Co Ltd, Zhongshan 528451, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Yuanpeng,Guo, Xiaoqi,Zhang, Wenying,et al. Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium[J]. INDUSTRIAL CROPS AND PRODUCTS,2022,189. |
| APA | Hao, Yuanpeng.,Guo, Xiaoqi.,Zhang, Wenying.,Xia, Fei.,Yang, Evan.,...&Shi, Lei.(2022).Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium.INDUSTRIAL CROPS AND PRODUCTS,189. |
| MLA | Hao, Yuanpeng,et al."Label-free quantitative proteomics reveals the antibacterial mechanism of rosemary essential oil against Salmonella enterica serovar Typhimurium".INDUSTRIAL CROPS AND PRODUCTS 189(2022). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。