New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS
文献类型：期刊论文
| 作者 | Sun, Shanshan4; Wang, Shujuan3; Li, Shanshan4; Wei, Liping3; Wang, Xiaohan4; Wang, Yizhou4; Li, Bing4; Hu, Yang1; Wang, Liangsheng4
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| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2022 |
| 卷号 | 1212 |
| 关键词 | Phytosterol Paeonia ostii 'Fengdan' Pollen Anther wall Gas chromatography-mass spectrometry (GC-MS) |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2022.339891 |
| 文献子类 | Article |
| 英文摘要 | Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') by gas chromatography-mass spectrometry. The result indicated that the contents of phytosterols in pollen (3390.02 mg/100 g DW) and anther wall (929.70 mg/100 g DW) of P. ostii 'Fengdan' were relatively high. Meanwhile, eleven phytosterols were identified, among which five phytosterols were first identified in tree peony pollen. Moreover, the effects of wall breaking and different developmental stages on phytosterol contents of pollen and anther wall of P. ostii 'Fengdan' were also discussed. The result showed that wall breaking was beneficial to sterol detection of pollen, but not necessary for anther wall. For the best harvest time, the stage of S2 had the highest content of phytosterols. In conclusion, this study successfully establishes an analytical method for phytosterols by gas chromatography-mass spectrometry, and lays a foundation for the development and utilization of pollen and anther wall of P. ostii 'Fengdan' in functional foods and pharmaceutical industry. |
| 学科主题 | Chemistry, Analytical |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1873-4324 |
| WOS关键词 | GAS-CHROMATOGRAPHY ; BEE POLLEN ; OILS ; PHYTOSTANOLS ; EXTRACTION ; FRACTION ; ESTERS ; FOODS ; SEEDS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000830632800001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China [32030095] ; Science and Technology Project of Lu'an Group [2019C062] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28971]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Shennong Zhihua Biotechnol Shanxi Co Ltd, Changzhi 046011, Peoples R China 2.Inner Mongolia Agr Univ, Coll Desert Management, Hohhot 010018, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Shanshan,Wang, Shujuan,Li, Shanshan,et al. New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS[J]. ANALYTICA CHIMICA ACTA,2022,1212. |
| APA | Sun, Shanshan.,Wang, Shujuan.,Li, Shanshan.,Wei, Liping.,Wang, Xiaohan.,...&Wang, Liangsheng.(2022).New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS.ANALYTICA CHIMICA ACTA,1212. |
| MLA | Sun, Shanshan,et al."New insights on the analysis of phytosterols in pollen and anther wall of tree peony (Paeonia ostii 'Fengdan') revealed by GC-MS/MS".ANALYTICA CHIMICA ACTA 1212(2022). |
入库方式： OAI收割
来源：植物研究所
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