Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads
文献类型：期刊论文
| 作者 | Xing, Mengyang2; Chen, Yong; Li, Boqiang; Tian, Shiping2
|
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2022 |
| 卷号 | 377 |
| 关键词 | Patulin degradation Pseudomonas aeruginosa Immobilized enzyme Calcium alginate beads Apple juice |
| ISSN号 | 0308-8146 |
| DOI | 10.1016/j.foodchem.2021.131973 |
| 文献子类 | Article |
| 英文摘要 | Patulin is a toxic secondary metabolite produced by several moulds, which contaminates fruits and their products posing serious threats to human health. Though several microorganisms and enzymes have been reported to effectively degrade patulin, separation of them from fruit juice challenges the commercial applications. Here, a Pseudomonas aeruginosa strain TF-06 was isolated, its patulin degradation mechanism and optimum conditions for enzyme immobilization were investigated. The results indicated that TF-06 could degrade patulin into noncytotoxic E/Z-ascladiol mainly by the activity of intracellular enzymes. For easy separation of enzymes, calcium alginate was selected for immobilization of intracellular enzymes from TF-06. The immobilized enzyme beads were effective in detoxification of patulin in apple juice. The mitigation rate was reached 95%, while there was no negative effect on juice quality. The study provides a promising way to resolve the issue of enzyme separation during mycotoxin biological detoxification in fruit juice. |
| 学科主题 | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1873-7072 |
| WOS关键词 | MYCOTOXIN PATULIN ; BIODEGRADATION ; INSIGHTS ; CELLS ; JUICE ; SOIL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000740932400006 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA26040202] ; National Natural Science Foundation of China [31930086] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29024]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing, Mengyang,Chen, Yong,Li, Boqiang,et al. Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads[J]. FOOD CHEMISTRY,2022,377. |
| APA | Xing, Mengyang,Chen, Yong,Li, Boqiang,&Tian, Shiping.(2022).Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads.FOOD CHEMISTRY,377. |
| MLA | Xing, Mengyang,et al."Highly efficient removal of patulin using immobilized enzymes of Pseudomonas aeruginosa TF-06 entrapped in calcium alginate beads".FOOD CHEMISTRY 377(2022). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。