Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening
文献类型：期刊论文
| 作者 | Wang, Yuying2; Kong, Lingxi1; Wang, Weihao2; Qin, Guozheng1,2
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| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2023 |
| 卷号 | 74期号:1页码:214-232 |
| 关键词 | Fruit ripening protein post-translational modification strawberry ubiquitination ubiquitinome ubiquitin-proteasome system (UPS) |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erac400 |
| 文献子类 | Article |
| 英文摘要 | Using ubiquitinome analysis we unravel several hundreds of ubiquitination sites on proteins in strawberry fruit, and identify a RING-type E3 ligase, FaBRIZ, which functions as an important negative regulator of ripening. Ubiquitination is an important post-translational modification that mediates protein degradation in eukaryotic cells, participating in multiple biological processes. However, the profiling of protein ubiquitination and the function of this crucial modification in fruit ripening remain largely unknown. In this study, we found that suppression of proteasome by the inhibitor MG132 retarded strawberry fruit ripening. Using K-e-GG antibody enrichment combined with high-resolution mass spectrometry, we performed a comprehensive ubiquitinome analysis in strawberry fruit. We identified 2947 ubiquitination sites for 2878 peptides within 1487 proteins, which are involved in a variety of cellular functions. The lysine at position 48 (K48)-linked poly-ubiquitin chains appeared to be the most prevalent type of modification among the identified ubiquitinated proteins. A large number of ubiquitination sites exhibited altered ubiquitination levels after proteasome inhibition, including those within ripening-related proteins associated with sugar and acid metabolism, cell wall metabolism, anthocyanin synthesis, and ABA biosynthesis and signalling. We further demonstrated that FaBRIZ, a RING-type E3 ligase, functions as a negative regulator of ripening in strawberry fruit. Our findings highlight the critical regulatory roles of protein ubiquitination in fruit ripening. The ubiquitinome data provide a basis for further exploration of the function of ubiquitination on specific proteins. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | TRANSCRIPTION FACTOR ; PROTEIN-DEGRADATION ; GENE-EXPRESSION ; TOMATO ; PROTEASOME ; ETHYLENE ; DEHYDROGENASE ; BIOSYNTHESIS ; INVOLVEMENT ; INHIBITOR |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000879103300001 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China [31925035, 32072636] ; National Key Research and Development Program [2018YFD1000200] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29088]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.China Natl Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuying,Kong, Lingxi,Wang, Weihao,et al. Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening[J]. JOURNAL OF EXPERIMENTAL BOTANY,2023,74(1):214-232. |
| APA | Wang, Yuying,Kong, Lingxi,Wang, Weihao,&Qin, Guozheng.(2023).Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening.JOURNAL OF EXPERIMENTAL BOTANY,74(1),214-232. |
| MLA | Wang, Yuying,et al."Global ubiquitinome analysis reveals the role of E3 ubiquitin ligase FaBRIZ in strawberry fruit ripening".JOURNAL OF EXPERIMENTAL BOTANY 74.1(2023):214-232. |
入库方式： OAI收割
来源：植物研究所
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