Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea
文献类型：期刊论文
| 作者 | Zhuo, Ruiling1,3; Li, Guangjin1,3; Peng, Huaimin; Zong, Yuanyuan; Wang, Xiao; Lu, Shasha1,3; Chen, Yong1; Zhang, Zhanquan1; Tian, Shiping1,3; Li, Boqiang1 |
| 刊名 | POSTHARVEST BIOLOGY AND TECHNOLOGY
 |
| 出版日期 | 2023 |
| 卷号 | 197 |
| ISSN号 | 0925-5214 |
| 关键词 | Ambient pH Botrytis cinerea PacC Polyketide synthases Secondary metabolism |
| DOI | 10.1016/j.postharvbio.2022.112219 |
| 文献子类 | Article |
| 英文摘要 | Botrytis cinerea is one of the most important postharvest pathogens and causes huge economic losses worldwide. Ambient pH is a critical factor influencing virulence of B. cinerea. In this study, BcpacC, a crucial gene encoding a pH-responsive transcription factor, was knocked out in B. cinerea strain B05.10. Deletion of BcpacC resulted in pH-dependent reduction in mycelial growth, sporulation, and virulence in apple and tomato fruit, and romaine lettuce leaves. An iTRAQ based proteomic analysis identified 70 differential proteins involved in various biological processes between wildtype (WT) and Delta BcpacC mutant under pH 6 condition, including 18 proteins related to secondary metabolism. Gene expression analysis indicates that 37 of all the 42 secondary metabolism key enzyme encoding genes were regulated by BcPacC. Further, three PacC-regulated polyketide synthase encoding genes, Bcpks4, Bcpks5 and Bcpks11, were knocked out and first proved to contribute to sporulation and virulence in B. cinerea. Our results revealed that the extensive regulation of BcPacC on secondary metabolism might play crucial roles in development and pathogenicity of B. cinerea during adaption to ambient pH. |
| 学科主题 | Agronomy ; Food Science & Technology ; Horticulture |
| 电子版国际标准刊号 | 1873-2356 |
| 出版地 | AMSTERDAM |
| WOS关键词 | AMBIENT PH ; POLYKETIDE SYNTHASE ; GENE-EXPRESSION ; BIOSYNTHESIS ; PATHOGENICITY ; SECRETION ; GROWTH ; FUNGI ; ENVIRONMENT ; PATHOTYPE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000904084400002 |
| 资助机构 | National Natural Science Foundation of China [32072273, 31722043, 31930086] ; National Key Research and Development Program of China [2021YFD2100501] ; Youth Innovation Promotion Association, CAS [Y201919] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/29140]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Natl Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhuo, Ruiling,Li, Guangjin,Peng, Huaimin,et al. Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2023,197. |
| APA | Zhuo, Ruiling.,Li, Guangjin.,Peng, Huaimin.,Zong, Yuanyuan.,Wang, Xiao.,...&Li, Boqiang.(2023).Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea.POSTHARVEST BIOLOGY AND TECHNOLOGY,197. |
| MLA | Zhuo, Ruiling,et al."Extensive regulation of pH-responsive transcription factor PacC on secondary metabolism contributes to development and virulence of Botrytis cinerea".POSTHARVEST BIOLOGY AND TECHNOLOGY 197(2023). |
入库方式： OAI收割
来源：植物研究所
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