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First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung

文献类型：期刊论文

作者Feng, Xin2,3; Gao, Pengli1,4,5,6; Li, Yabin7; Hui, Hui2,3; Jiang, Jingying5,6,9; Xie, Fei7,10; Tian, Jie2,5,6,8,9
刊名BIOENGINEERING & TRANSLATIONAL MEDICINE
出版日期2023-11-29
页码10
关键词in vivo lung injury magnetic particle imaging quantitative vascular leakage
DOI10.1002/btm2.10626
通讯作者Jiang, Jingying(jingyingjiang@buaa.edu.cn) ; Xie, Fei(xiefei0522@163.com) ; Tian, Jie(tian@ieee.org)
英文摘要Increased pulmonary vascular permeability is a characteristic feature of lung injury. However, there are no established methods that allow the three-dimensional visualization and quantification of pulmonary vascular permeability in vivo. Evans blue extravasation test and total protein test of bronchoalveolar lavage fluid (BALF) are permeability assays commonly used in research settings. However, they lack the ability to identify the spatial and temporal heterogeneity of endothelial barrier disruption, which is typical in lung injuries. Magnetic resonance (MR) and near-infrared (NIR) imaging have been proposed to image pulmonary permeability, but suffer from limited sensitivity and penetration depth, respectively. In this study, we report the first use of magnetic particle imaging (MPI) to assess pulmonary vascular leakage noninvasively in vivo in mice. A dextran-coated superparamagnetic iron oxide (SPIO), synomag (R), was employed as the imaging tracer, and pulmonary SPIO extravasation was imaged and quantified to evaluate the vascular leakage. Animal models of acute lung injury and pulmonary fibrosis (PF) were used to validate the proposed method. MPI sensitively detected the SPIO extravasation in both acutely injured and fibrotic lungs in vivo, which was confirmed by ex vivo imaging and Prussian blue staining. Moreover, 3D MPI illustrated the spatial heterogeneity of vascular leakage, which correlated well with CT findings. Based on the in vivo 3D MPI images, we defined the SPIO extravasation index (SEI) to quantify the vascular leakage. A significant increase in SEI was observed in the injured lungs, in consistent with the results obtained via ex vivo permeability assays. Overall, our results demonstrate that 3D quantitative MPI serves as a useful tool to examine pulmonary vascular integrity in vivo, which shows promise for future clinical translation.
WOS关键词MODELS
资助项目Natural Science Foundation of Beijing Municipality
WOS研究方向Biotechnology & Applied Microbiology ; Engineering ; Pharmacology & Pharmacy
语种英语
出版者WILEY
WOS记录号WOS:001115271700001
资助机构Natural Science Foundation of Beijing Municipality
源URL[http://ir.ia.ac.cn/handle/173211/55101]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Jiang, Jingying; Xie, Fei; Tian, Jie
作者单位1.Beihang Univ, Sch Engn Med, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing, Peoples R China
4.Beihang Univ, Sch Biol Sci & Med Engn, Beijing, Peoples R China
5.Beihang Univ, Key Lab Big Data Based Precis Med, Minist Ind & Informat Technol, Beijing, Peoples R China
6.Beihang Univ, Sch Engn Med, Beijing, Peoples R China
7.Chinese Peoples Liberat Army Gen Hosp, Coll Pulm & Crit Care Med, Beijing, Peoples R China
8.Beihang Univ, Sch Biol Sci & Med Engn, 37,Xueyuan Rd, Beijing 100191, Peoples R China
9.Beihang Univ, Sch Engn Med, 37,Xueyuan Rd, Beijing 100191, Peoples R China
10.Chinese Peoples Liberat Army Gen Hosp, Coll Pulm & Crit Care Med, 28 Fuxing Rd, Beijing 100853, Peoples R China
推荐引用方式
GB/T 7714		 Feng, Xin,Gao, Pengli,Li, Yabin,et al. First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung[J]. BIOENGINEERING & TRANSLATIONAL MEDICINE,2023:10.
APA Feng, Xin.,Gao, Pengli.,Li, Yabin.,Hui, Hui.,Jiang, Jingying.,...&Tian, Jie.(2023).First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung.BIOENGINEERING & TRANSLATIONAL MEDICINE,10.
MLA Feng, Xin,et al."First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung".BIOENGINEERING & TRANSLATIONAL MEDICINE (2023):10.

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来源：自动化研究所

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