Systematical targeted multicomponent characterization and comparison of Arnebiae Radix and its three confusing species by offline two-dimensional liquid chromatography/LTQ-Orbitrap mass spectrometry
文献类型：期刊论文
| 作者 | Sha, Fei1; Zhang, Jianqing3; Yang, Huanya2; Hu, Yunshu2; Wei, Wenlong3; Wang, Cuicui3; Li, Xiaolan3; Shen, Xuanjing3; An, Yaling3; Li, Jiayuan3 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2023-12-07 |
| 页码 | 13 |
| 关键词 | Arnebiae Radix Two-dimensional liquid chromatography Mass defect filter Precursor ion list Components characterization |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-023-05067-x |
| 通讯作者 | Guo, Dean(daguo@simm.ac.cn) |
| 英文摘要 | Arnebiae Radix, commonly known as "Zicao," can be easily confused with other compounding species, posing challenges for its clinical use. Here, we developed a comprehensive strategy to systematically characterize the diverse components across Arnebiae Radix and its three confusing species. First, an offline two-dimensional liquid chromatography (2D-LC) system integrating hydrophilic interaction chromatography (HILIC) and reverse phase (RP) separations was established, enabling effective separation and detection of more trace constituents. Second, a polygonal mass defect filtering (MDF) workflow was implemented to screen target ions and generate a precursor ion list (PIL) to guide multistage mass (MSn) data acquisition. Third, a three-step characterization strategy utilizing diagnostic ions and neutral losses was developed for rapid determination of molecular formulas, structure classes, and compound identification. This approach enabled systematic characterization of Arnebiae Radix and its three confusing species, with 437 components characterized including 112 shikonins, 22 shikonfurans, 144 phenolic acids, 131 glycosides, 18 flavonoids, and 10 other compounds. Additionally, 361, 230, 340, and 328 components were identified from RZC, YZC, DZC, and ZZC, respectively, with 142 common components and 30 characteristic components that may serve as potential markers for distinguishing the four species. In summary, this is the first comprehensive characterization and comparison of the phytochemical profiles of Arnebiae Radix and its three confusing species, advancing our understanding of this herbal medicine for quality control. |
| WOS关键词 | RAPID IDENTIFICATION ; PHENOLIC-ACIDS ; FLAVONOIDS ; ALKALOIDS ; METABOLITES ; STRATEGY |
| 资助项目 | Shanghai Sailing Program[21YF1455800] ; Qi-Huang Chief Scientist Project of National Administration of Traditional Chinese Medicine (2020) ; Science and Technology Major Project of Inner Mongolia[2021ZD0017] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001117772900002 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/308517]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, Dean |
| 作者单位 | 1.Hangzhou Normal Univ, Sch Pharm, Hangzhou 311121, Zhejiang, Peoples R China 2.Nanjing Univ Chinese Med, Sch Pharm, Nanjing, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sha, Fei,Zhang, Jianqing,Yang, Huanya,et al. Systematical targeted multicomponent characterization and comparison of Arnebiae Radix and its three confusing species by offline two-dimensional liquid chromatography/LTQ-Orbitrap mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2023:13. |
| APA | Sha, Fei.,Zhang, Jianqing.,Yang, Huanya.,Hu, Yunshu.,Wei, Wenlong.,...&Guo, Dean.(2023).Systematical targeted multicomponent characterization and comparison of Arnebiae Radix and its three confusing species by offline two-dimensional liquid chromatography/LTQ-Orbitrap mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,13. |
| MLA | Sha, Fei,et al."Systematical targeted multicomponent characterization and comparison of Arnebiae Radix and its three confusing species by offline two-dimensional liquid chromatography/LTQ-Orbitrap mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2023):13. |
入库方式： OAI收割
来源：上海药物研究所
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