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The development of logic gate-based fluorescent probes that respond to intracellular hydrogen peroxide and pH in tandem

文献类型：期刊论文

作者Han, Hai-Hao1,4,8; Liu, Meng-Jiao1,8; Zhang, Weijian7; Sun, Lu-Lu6; Ma, Xiuhua7; Qiao, Hongjin7,8; Sun, Shasha7; Yang, Jianming7; Chai, Xianzhi2,7; Wu, Zhenyong1
刊名TALANTA
出版日期2024-04-01
卷号270页码:8
ISSN号0039-9140
关键词Reactive oxygen species (ROS) pH Logic gate Fluorescent probe Cell imaging
DOI10.1016/j.talanta.2023.125526
通讯作者Chai, Xianzhi(chaixianzhi@163.com) ; Wu, Zhenyong(wuzhenyong@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn)
英文摘要Logic gate-based fluorescent probes are powerful tools for the discriminative sensing of multiple signaling molecules that are expressed in concert during the progression of many diseases such as inflammation, cancer, aging, and other disorders. To achieve logical sensing, multiple functional groups are introduced to the different substitution sites of a single fluorescent dye, which increases the complexity of chemical synthesis. Herein, we report a simple strategy that incorporates just one responsive unit into a hemicyanine dye achieving the logic gate-based sensing of two independent analytes. We introduce boronic acid to hemicyanine to quench the fluorescence, and in the presence of hydrogen peroxide (H2O2), the fluorescence is recovered due to removal of the boronate. Interestingly, the subsequent decrease in pH turned the red fluorescence of hemicyanine to green emissive because of protonation of the phenolic alcohol. This unique feature of the probe enables us to construct "INHIBIT" and "AND" logical gates for the accurate measuring of intracellular H2O2 and acidic pH in tandem. This study offers insight into the simple construction of logic-gate based fluorescent probes for the tandem sensing of multiple analytes that are correlatively produced during disease progression.
WOS关键词AUTOPHAGY
资助项目National Natural Science Foundation of China[22107029] ; National Natural Science Foundation of China[22308134] ; National Natural Science Foundation of China[22377135] ; National Natural Science Foundation of China[21807050] ; National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; Natural Science Foundation of Jiangsu Province[BK20220644] ; Natural Science Foundation of Jiangsu Province[BK20210894] ; Fundamental Research Projects of Science & Technology Innovation and Development Plan in Yantai City[2023JCYJ059] ; Shandong Laboratory Program[SYS202205] ; Shanghai Municipal Science and Technology Major Project[2018SHZDZX03] ; Fundamental Research Funds for the Central Universities[222201717003] ; Programme of Introducing Talents of Discipline to Universities[B16017] ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering
WOS研究方向Chemistry
语种英语
出版者ELSEVIER
WOS记录号WOS:001136984000001
源URL[http://119.78.100.183/handle/2S10ELR8/308571]  
专题中国科学院上海药物研究所
通讯作者Chai, Xianzhi; Wu, Zhenyong; He, Xiao-Peng
作者单位1.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
2.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China
3.Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
5.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat & Joint Int Re, 130 Meilong Rd, Shanghai 200237, Peoples R China
6.Chinese Acad Sci, Mol Imaging Ctr, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
7.Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhenjiang 212100, Jiangsu, Peoples R China
8.Ludong Univ, Sch Life Sci, Yantai 264025, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Han, Hai-Hao,Liu, Meng-Jiao,Zhang, Weijian,et al. The development of logic gate-based fluorescent probes that respond to intracellular hydrogen peroxide and pH in tandem[J]. TALANTA,2024,270:8.
APA Han, Hai-Hao.,Liu, Meng-Jiao.,Zhang, Weijian.,Sun, Lu-Lu.,Ma, Xiuhua.,...&He, Xiao-Peng.(2024).The development of logic gate-based fluorescent probes that respond to intracellular hydrogen peroxide and pH in tandem.TALANTA,270,8.
MLA Han, Hai-Hao,et al."The development of logic gate-based fluorescent probes that respond to intracellular hydrogen peroxide and pH in tandem".TALANTA 270(2024):8.

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来源：上海药物研究所

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