Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition
文献类型：期刊论文
| 作者 | Chen, Jiali1,2; Wang, Yifan2; Lin, Shuru2; Yu, Quanxiang2; Qi, Zhengfei2; Jiang, Wenqi2; Zhao, Qiang1,2 ; Fu, Qingshan Bill1,2
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2024-01-29 |
| 卷号 | 694页码:5 |
| ISSN号 | 0006-291X |
| 关键词 | SpoVM SpoIVA Purification Bicelles Interaction SpoVMP9A |
| DOI | 10.1016/j.bbrc.2023.149395 |
| 通讯作者 | Zhao, Qiang(zhaoq@simm.ac.cn) ; Fu, Qingshan Bill(fuqingshan@simm.ac.cn) |
| 英文摘要 | SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVMP9A were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving NiNTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVMP9A) and SpoIVA in Bicelle conditions. |
| WOS关键词 | MORPHOGENETIC PROTEIN ; SPORULATION ; COAT ; LOCALIZATION ; GENE |
| 资助项目 | National Natural Science Foundation of China[32271320] ; Creative Research Group of Zhongshan City (Lingnan Pharmaceutical Research and Innovation team)[CXTD2022011] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:001144855200001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/308663]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhao, Qiang; Fu, Qingshan Bill |
| 作者单位 | 1.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jiali,Wang, Yifan,Lin, Shuru,et al. Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2024,694:5. |
| APA | Chen, Jiali.,Wang, Yifan.,Lin, Shuru.,Yu, Quanxiang.,Qi, Zhengfei.,...&Fu, Qingshan Bill.(2024).Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,694,5. |
| MLA | Chen, Jiali,et al."Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 694(2024):5. |
入库方式： OAI收割
来源：上海药物研究所
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