中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics

文献类型：期刊论文

作者Ma, Wenjing1,2; Xu, Zhuojia1,2; Jiang, Yuhan2; Liu, Jialin2; Xu, Dandan3; Huang, Wei2,3; Li, Tiehai1,2
刊名ADVANCED SCIENCE
出版日期2023-08-26
页码10
关键词carbohydrates enzyme-catalyzed synthesis functional glycomics glycan microarray IgG N-glycan library
DOI10.1002/advs.202303832
通讯作者Li, Tiehai(tiehaili@simm.ac.cn)
英文摘要N-Glycosylation, a main post-translational modification of Immunoglobulin G (IgG), plays a significant role in modulating the immune functions of IgG. However, the precise function elucidation of IgG N-glycosylation remains impeded due to the obstacles in obtaining comprehensive and well-defined N-glycans. Here, an easy-to-implement divergent approach is described to synthesize a 64-membered IgG N-glycan library covering all possible biantennary and bisected N-glycans by reprogramming biosynthetic assembly lines based on the inherent branch selectivity and substrate specificity of enzymes. The unique binding specificities of 64 N-glycans with different proteins are deciphered by glycan microarray technology. This unprecedented collection of synthetic IgG N-glycans can serve as standards for N-glycan structure identification in complex biological samples and the microarray data enrich N-glycan glycomics to facilitate biomedical applications.
WOS关键词CHEMOENZYMATIC SYNTHESIS ; SIALYLGLYCOPEPTIDE SGP ; BRANCH SPECIFICITY ; SCALE PRODUCTION ; GLYCOSYLATION ; OLIGOSACCHARIDES ; TRIANTENNARY ; BIANTENNARY ; STRATEGY ; BI
资助项目National Natural Science Foundation of China ; Science and Technology Commission of Shanghai Municipality[20ZR1467900] ; CAS Pioneer Talent Program ; Shanghai Municipal Science and Technology Major Project ; [22077130]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
出版者WILEY
WOS记录号WOS:001153591800001
源URL[http://119.78.100.183/handle/2S10ELR8/308948]  
专题中国科学院上海药物研究所
通讯作者Li, Tiehai
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
3.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Wenjing,Xu, Zhuojia,Jiang, Yuhan,et al. Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics[J]. ADVANCED SCIENCE,2023:10.
APA Ma, Wenjing.,Xu, Zhuojia.,Jiang, Yuhan.,Liu, Jialin.,Xu, Dandan.,...&Li, Tiehai.(2023).Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.ADVANCED SCIENCE,10.
MLA Ma, Wenjing,et al."Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics".ADVANCED SCIENCE (2023):10.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。