Chemical Synthesis Creates Single Glycoforms of the Ectodomain of Herpes Simplex Virus-1 Glycoprotein D
文献类型：期刊论文
| 作者 | Zhao, Jie1; Liu, Xinliang1; Liu, Jialin4; Ye, Farong1; Wei, Bingcheng1; Deng, Minggang3; Li, Tiehai4; Huang, Ping1; Wang, Ping1,2 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2023-12-20 |
| 卷号 | 146期号:4页码:2615-2623 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.3c11543 |
| 通讯作者 | Li, Tiehai(tiehaili@simm.ac.cn) ; Huang, Ping(hp158@sjtu.edu.cn) ; Wang, Ping(wangp1@sjtu.edu.cn) |
| 英文摘要 | Herpes simplex virus-1 (HSV-1) utilizes multiple viral surface glycoproteins to trigger virus entry and fusion. Among these glycoproteins, glycoprotein D (gD) functions as a receptor-binding protein, which makes it an attractive target for the development of vaccines against HSV-1 infection. Several recombinant gD subunit vaccines have been investigated in both preclinical and clinical phases with varying degrees of success. It is fundamentally critical to explore the functions of gD glycans. In light of this, we report an efficient synthetic platform to construct glycosylated gDs bearing homogeneous glycans at N94 and N121. The oligosaccharides were prepared by enzymatic synthesis and conjugated to peptidyl sectors. The glycoproteins were constructed via a combination of 7-(piperazin-1-yl)-2-(methyl)-quinolinyl (PPZQ)-assisted expressed protein ligation and beta-mercapto amino acid-assisted-desulfurization strategies. Biological studies showed that synthetic gDs exhibited potent in vivo activity in mice. |
| WOS关键词 | LINKED OLIGOSACCHARIDES ; LIGATION ; PROTEIN ; TYPE-1 ; DESULFURIZATION ; CYSTEINE ; GLYCOPEPTIDES ; EXPRESSION ; INFECTION ; PEPTIDES |
| 资助项目 | China Postdoctoral Science Foundation[22225701] ; National Science Fund for Distinguished Young Scholars[22077080] ; National Science Fund for Distinguished Young Scholars[92253302] ; National Science Fund for Distinguished Young Scholars[22077130] ; National Science Fund for Distinguished Young Scholars[22275122] ; National Natural Science Foundation of China[21TQ1400210] ; Shanghai Pilot Program for Basic Research-Shanghai Jiao Tong University[2023M732214] ; China Postdoctoral Science Foundation[20230102] ; Interdisciplinary Program of Shanghai Jiao Tong University[2021Szvup077] ; Special Projects of the Central Government in Guidance of Local Science and Technology Development |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:001155532300001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/309063]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Tiehai; Huang, Ping; Wang, Ping |
| 作者单位 | 1.Zhangjiang Inst Adv Study, Ctr Chem Glycobiol, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn,Shanghai Key Lab Mol Engn Ch, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jie,Liu, Xinliang,Liu, Jialin,et al. Chemical Synthesis Creates Single Glycoforms of the Ectodomain of Herpes Simplex Virus-1 Glycoprotein D[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2023,146(4):2615-2623. |
| APA | Zhao, Jie.,Liu, Xinliang.,Liu, Jialin.,Ye, Farong.,Wei, Bingcheng.,...&Wang, Ping.(2023).Chemical Synthesis Creates Single Glycoforms of the Ectodomain of Herpes Simplex Virus-1 Glycoprotein D.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,146(4),2615-2623. |
| MLA | Zhao, Jie,et al."Chemical Synthesis Creates Single Glycoforms of the Ectodomain of Herpes Simplex Virus-1 Glycoprotein D".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 146.4(2023):2615-2623. |
入库方式： OAI收割
来源：上海药物研究所
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